Experiment: nitrite (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06445 and SGL_RS06450 are separated by 111 nucleotides SGL_RS06450 and SGL_RS06455 are separated by 156 nucleotides SGL_RS06455 and SGL_RS06460 overlap by 4 nucleotides SGL_RS06460 and SGL_RS06465 are separated by 3 nucleotides
SGL_RS06445: SGL_RS06445 - DUF3782 domain-containing protein, at 978,941 to 979,573
_RS06445
SGL_RS06450: SGL_RS06450 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 979,685 to 980,170
_RS06450
SGL_RS06455: SGL_RS06455 - guanitoxin biosynthesis MATE family efflux transporter GntT, at 980,327 to 981,694
_RS06455
SGL_RS06460: SGL_RS06460 - fatty acid desaturase, at 981,691 to 982,629
_RS06460
SGL_RS06465: SGL_RS06465 - hypothetical protein, at 982,633 to 982,920
_RS06465
Position (kb) 980
981
982 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 979.501 kb on - strand, within SGL_RS06445 at 980.166 kb on + strand at 980.266 kb on + strand at 980.313 kb on + strand at 980.321 kb on - strand at 980.321 kb on - strand at 980.339 kb on - strand at 980.341 kb on + strand at 980.396 kb on - strand at 980.423 kb on + strand at 980.605 kb on + strand, within SGL_RS06455 at 980.656 kb on + strand, within SGL_RS06455 at 980.743 kb on + strand, within SGL_RS06455 at 980.866 kb on - strand, within SGL_RS06455 at 980.940 kb on - strand, within SGL_RS06455 at 981.089 kb on + strand, within SGL_RS06455 at 981.322 kb on - strand, within SGL_RS06455 at 981.334 kb on + strand, within SGL_RS06455 at 981.512 kb on + strand, within SGL_RS06455 at 981.600 kb on + strand at 981.697 kb on - strand at 982.453 kb on - strand, within SGL_RS06460 at 982.495 kb on + strand, within SGL_RS06460 at 982.626 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite (N) remove 979,501 - SGL_RS06445 0.88 -2.9 980,166 + -1.7 980,266 + -2.4 980,313 + -4.6 980,321 - -5.7 980,321 - -3.3 980,339 - -4.5 980,341 + -4.5 980,396 - -3.3 980,423 + -6.4 980,605 + SGL_RS06455 0.20 -5.2 980,656 + SGL_RS06455 0.24 -5.2 980,743 + SGL_RS06455 0.30 -5.6 980,866 - SGL_RS06455 0.39 -3.9 980,940 - SGL_RS06455 0.45 -6.2 981,089 + SGL_RS06455 0.56 -5.1 981,322 - SGL_RS06455 0.73 -3.0 981,334 + SGL_RS06455 0.74 -4.3 981,512 + SGL_RS06455 0.87 -4.5 981,600 + -2.8 981,697 - +0.7 982,453 - SGL_RS06460 0.81 -2.8 982,495 + SGL_RS06460 0.86 -2.8 982,626 - -0.4
Or see this region's nucleotide sequence