Experiment: nitrite (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05400 and SGL_RS05405 are separated by 555 nucleotides SGL_RS05405 and SGL_RS05410 are separated by 48 nucleotides SGL_RS05410 and SGL_RS05415 overlap by 50 nucleotides
SGL_RS05400: SGL_RS05400 - Crp/Fnr family transcriptional regulator, at 754,877 to 755,497
_RS05400
SGL_RS05405: SGL_RS05405 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 756,053 to 757,195
_RS05405
SGL_RS05410: SGL_RS05410 - type II secretion system protein, at 757,244 to 757,720
_RS05410
SGL_RS05415: SGL_RS05415 - prepilin-type N-terminal cleavage/methylation domain-containing protein, at 757,671 to 758,753
_RS05415
Position (kb)
756
757
758 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 755.149 kb on - strand, within SGL_RS05400 at 755.273 kb on - strand, within SGL_RS05400 at 755.790 kb on - strand at 755.969 kb on + strand at 756.186 kb on + strand, within SGL_RS05405 at 756.194 kb on - strand, within SGL_RS05405 at 756.198 kb on + strand, within SGL_RS05405 at 756.324 kb on + strand, within SGL_RS05405 at 756.386 kb on - strand, within SGL_RS05405 at 756.415 kb on - strand, within SGL_RS05405 at 756.570 kb on - strand, within SGL_RS05405 at 756.727 kb on - strand, within SGL_RS05405 at 756.733 kb on + strand, within SGL_RS05405 at 756.802 kb on + strand, within SGL_RS05405 at 757.022 kb on + strand, within SGL_RS05405 at 757.022 kb on + strand, within SGL_RS05405 at 757.065 kb on - strand, within SGL_RS05405 at 757.387 kb on - strand, within SGL_RS05410 at 757.504 kb on - strand, within SGL_RS05410 at 758.029 kb on + strand, within SGL_RS05415
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite (N) remove 755,149 - SGL_RS05400 0.44 -1.9 755,273 - SGL_RS05400 0.64 -3.6 755,790 - -1.5 755,969 + -1.9 756,186 + SGL_RS05405 0.12 -2.0 756,194 - SGL_RS05405 0.12 -2.0 756,198 + SGL_RS05405 0.13 -1.5 756,324 + SGL_RS05405 0.24 -1.3 756,386 - SGL_RS05405 0.29 -0.4 756,415 - SGL_RS05405 0.32 -2.8 756,570 - SGL_RS05405 0.45 -1.7 756,727 - SGL_RS05405 0.59 -2.4 756,733 + SGL_RS05405 0.59 -2.7 756,802 + SGL_RS05405 0.66 -2.0 757,022 + SGL_RS05405 0.85 -1.8 757,022 + SGL_RS05405 0.85 -1.0 757,065 - SGL_RS05405 0.89 -1.0 757,387 - SGL_RS05410 0.30 -1.4 757,504 - SGL_RS05410 0.55 -0.2 758,029 + SGL_RS05415 0.33 -3.1
Or see this region's nucleotide sequence