Experiment: nitrite (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04330 and SGL_RS04335 are separated by 28 nucleotides SGL_RS04335 and SGL_RS04340 are separated by 210 nucleotides SGL_RS04340 and SGL_RS04345 are separated by 245 nucleotides
SGL_RS04330: SGL_RS04330 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 529,735 to 530,688
_RS04330
SGL_RS04335: SGL_RS04335 - GNAT family N-acetyltransferase, at 530,717 to 531,235
_RS04335
SGL_RS04340: SGL_RS04340 - type I glutamate--ammonia ligase, at 531,446 to 532,867
_RS04340
SGL_RS04345: SGL_RS04345 - response regulator, at 533,113 to 537,474
_RS04345
Position (kb) 531
532
533 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 530.518 kb on - strand, within SGL_RS04330 at 530.806 kb on + strand, within SGL_RS04335 at 530.806 kb on + strand, within SGL_RS04335 at 530.814 kb on - strand, within SGL_RS04335 at 530.895 kb on + strand, within SGL_RS04335 at 530.966 kb on + strand, within SGL_RS04335 at 530.974 kb on + strand, within SGL_RS04335 at 531.193 kb on - strand at 531.337 kb on - strand at 531.395 kb on + strand at 531.493 kb on + strand at 531.539 kb on - strand at 531.575 kb on + strand at 531.585 kb on - strand at 531.620 kb on - strand, within SGL_RS04340 at 531.664 kb on + strand, within SGL_RS04340 at 531.751 kb on + strand, within SGL_RS04340 at 531.751 kb on + strand, within SGL_RS04340 at 531.759 kb on - strand, within SGL_RS04340 at 531.764 kb on - strand, within SGL_RS04340 at 531.764 kb on - strand, within SGL_RS04340 at 531.779 kb on - strand, within SGL_RS04340 at 531.914 kb on + strand, within SGL_RS04340 at 531.960 kb on - strand, within SGL_RS04340 at 532.006 kb on + strand, within SGL_RS04340 at 532.093 kb on + strand, within SGL_RS04340 at 532.102 kb on - strand, within SGL_RS04340 at 532.281 kb on + strand, within SGL_RS04340 at 532.389 kb on - strand, within SGL_RS04340 at 532.436 kb on - strand, within SGL_RS04340 at 532.457 kb on + strand, within SGL_RS04340 at 532.515 kb on - strand, within SGL_RS04340 at 532.547 kb on + strand, within SGL_RS04340 at 532.584 kb on + strand, within SGL_RS04340 at 532.628 kb on + strand, within SGL_RS04340 at 532.715 kb on - strand, within SGL_RS04340 at 532.756 kb on + strand at 532.793 kb on + strand at 532.821 kb on - strand at 532.903 kb on - strand at 533.168 kb on + strand at 533.176 kb on - strand at 533.199 kb on - strand at 533.483 kb on + strand at 533.507 kb on - strand at 533.521 kb on + strand at 533.574 kb on - strand, within SGL_RS04345 at 533.614 kb on - strand, within SGL_RS04345 at 533.639 kb on + strand, within SGL_RS04345 at 533.715 kb on + strand, within SGL_RS04345 at 533.723 kb on - strand, within SGL_RS04345
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite (N) remove 530,518 - SGL_RS04330 0.82 -1.8 530,806 + SGL_RS04335 0.17 +0.5 530,806 + SGL_RS04335 0.17 +0.5 530,814 - SGL_RS04335 0.19 +0.9 530,895 + SGL_RS04335 0.34 +0.4 530,966 + SGL_RS04335 0.48 +0.2 530,974 + SGL_RS04335 0.50 +0.7 531,193 - -0.2 531,337 - -0.2 531,395 + -2.3 531,493 + -3.4 531,539 - -4.2 531,575 + -3.5 531,585 - -3.2 531,620 - SGL_RS04340 0.12 -1.4 531,664 + SGL_RS04340 0.15 -2.5 531,751 + SGL_RS04340 0.21 -1.5 531,751 + SGL_RS04340 0.21 -0.8 531,759 - SGL_RS04340 0.22 -1.5 531,764 - SGL_RS04340 0.22 -2.1 531,764 - SGL_RS04340 0.22 -2.4 531,779 - SGL_RS04340 0.23 -2.0 531,914 + SGL_RS04340 0.33 -1.9 531,960 - SGL_RS04340 0.36 -1.6 532,006 + SGL_RS04340 0.39 -2.0 532,093 + SGL_RS04340 0.45 -2.7 532,102 - SGL_RS04340 0.46 -1.8 532,281 + SGL_RS04340 0.59 -1.2 532,389 - SGL_RS04340 0.66 -4.4 532,436 - SGL_RS04340 0.70 -1.4 532,457 + SGL_RS04340 0.71 -0.6 532,515 - SGL_RS04340 0.75 -0.6 532,547 + SGL_RS04340 0.77 -0.9 532,584 + SGL_RS04340 0.80 -0.9 532,628 + SGL_RS04340 0.83 -2.5 532,715 - SGL_RS04340 0.89 -2.5 532,756 + -2.1 532,793 + +0.2 532,821 - -2.0 532,903 - -0.1 533,168 + -0.5 533,176 - -0.3 533,199 - -0.2 533,483 + +0.2 533,507 - -0.3 533,521 + +0.6 533,574 - SGL_RS04345 0.11 -0.7 533,614 - SGL_RS04345 0.11 -1.0 533,639 + SGL_RS04345 0.12 -0.2 533,715 + SGL_RS04345 0.14 +0.0 533,723 - SGL_RS04345 0.14 -0.0
Or see this region's nucleotide sequence