Experiment: nitrite (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00340 and SGL_RS00345 are separated by 362 nucleotides SGL_RS00345 and SGL_RS00350 are separated by 91 nucleotides SGL_RS00350 and SGL_RS00355 are separated by 120 nucleotides
SGL_RS00340: SGL_RS00340 - VOC family protein, at 73,728 to 74,147
_RS00340
SGL_RS00345: SGL_RS00345 - dihydrofolate reductase family protein, at 74,510 to 75,055
_RS00345
SGL_RS00350: SGL_RS00350 - Rpn family recombination-promoting nuclease/putative transposase, at 75,147 to 75,929
_RS00350
SGL_RS00355: SGL_RS00355 - ion channel, at 76,050 to 76,988
_RS00355
Position (kb) 74
75
76 Strain fitness (log2 ratio) -1
0
1 at 73.636 kb on + strand at 73.671 kb on + strand at 73.747 kb on - strand at 73.945 kb on - strand, within SGL_RS00340 at 74.420 kb on - strand at 74.803 kb on + strand, within SGL_RS00345 at 74.976 kb on - strand, within SGL_RS00345 at 74.986 kb on - strand, within SGL_RS00345 at 75.019 kb on + strand at 75.028 kb on - strand at 75.046 kb on - strand at 75.461 kb on + strand, within SGL_RS00350 at 75.484 kb on - strand, within SGL_RS00350
Per-strain Table
Position Strand Gene LocusTag Fraction nitrite (N) remove 73,636 + -0.3 73,671 + +0.3 73,747 - -0.0 73,945 - SGL_RS00340 0.52 +0.8 74,420 - +0.2 74,803 + SGL_RS00345 0.54 -0.7 74,976 - SGL_RS00345 0.85 +0.3 74,986 - SGL_RS00345 0.87 -0.8 75,019 + -0.1 75,028 - -0.1 75,046 - +0.4 75,461 + SGL_RS00350 0.40 +0.6 75,484 - SGL_RS00350 0.43 -0.5
Or see this region's nucleotide sequence