Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11970 and SGL_RS18940 are separated by 56 nucleotides SGL_RS18940 and SGL_RS11975 are separated by 101 nucleotides SGL_RS11975 and SGL_RS11980 are separated by 50 nucleotides SGL_RS11980 and SGL_RS11985 are separated by 268 nucleotides
SGL_RS11970: SGL_RS11970 - HNH endonuclease, at 2,138,074 to 2,138,439
_RS11970
SGL_RS18940: SGL_RS18940 - DUF433 domain-containing protein, at 2,138,496 to 2,138,621
_RS18940
SGL_RS11975: SGL_RS11975 - DUF2358 domain-containing protein, at 2,138,723 to 2,139,142
_RS11975
SGL_RS11980: SGL_RS11980 - phosphoglycerate kinase, at 2,139,193 to 2,140,395
_RS11980
SGL_RS11985: SGL_RS11985 - LysR family transcriptional regulator, at 2,140,664 to 2,141,593
_RS11985
Position (kb) 2139
2140
2141 Strain fitness (log2 ratio) -2
-1
0
1
2 at 2138.222 kb on + strand, within SGL_RS11970 at 2138.765 kb on + strand, within SGL_RS11975 at 2138.865 kb on - strand, within SGL_RS11975 at 2138.923 kb on + strand, within SGL_RS11975 at 2139.900 kb on - strand, within SGL_RS11980 at 2140.612 kb on + strand at 2140.620 kb on - strand at 2141.044 kb on - strand, within SGL_RS11985
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 2,138,222 + SGL_RS11970 0.40 +0.6 2,138,765 + SGL_RS11975 0.10 -0.1 2,138,865 - SGL_RS11975 0.34 -0.6 2,138,923 + SGL_RS11975 0.48 +0.1 2,139,900 - SGL_RS11980 0.59 -2.1 2,140,612 + +0.9 2,140,620 - +2.5 2,141,044 - SGL_RS11985 0.41 +1.1
Or see this region's nucleotide sequence