Experiment: high light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03000 and SGL_RS03005 are separated by 74 nucleotides SGL_RS03005 and SGL_RS03010 are separated by 107 nucleotides SGL_RS03010 and SGL_RS03015 are separated by 97 nucleotides SGL_RS03015 and SGL_RS03020 are separated by 100 nucleotides
SGL_RS03000: SGL_RS03000 - spore maturation protein, at 244,160 to 244,708
_RS03000
SGL_RS03005: SGL_RS03005 - nucleoside recognition domain-containing protein, at 244,783 to 245,394
_RS03005
SGL_RS03010: SGL_RS03010 - 4-alpha-glucanotransferase, at 245,502 to 247,019
_RS03010
SGL_RS03015: SGL_RS03015 - VOC family protein, at 247,117 to 247,569
_RS03015
SGL_RS03020: SGL_RS03020 - thiamine-phosphate kinase, at 247,670 to 248,713
_RS03020
Position (kb) 245
246
247
248 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1 at 244.540 kb on - strand, within SGL_RS03000 at 244.570 kb on - strand, within SGL_RS03000 at 245.163 kb on + strand, within SGL_RS03005 at 245.171 kb on - strand, within SGL_RS03005 at 245.406 kb on - strand at 245.426 kb on - strand at 245.468 kb on - strand at 245.601 kb on - strand at 245.620 kb on - strand at 245.779 kb on - strand, within SGL_RS03010 at 245.787 kb on - strand, within SGL_RS03010 at 245.833 kb on + strand, within SGL_RS03010 at 245.833 kb on + strand, within SGL_RS03010 at 245.841 kb on - strand, within SGL_RS03010 at 245.950 kb on + strand, within SGL_RS03010 at 246.097 kb on + strand, within SGL_RS03010 at 246.353 kb on - strand, within SGL_RS03010 at 246.353 kb on - strand, within SGL_RS03010 at 246.371 kb on - strand, within SGL_RS03010 at 246.560 kb on - strand, within SGL_RS03010 at 246.571 kb on + strand, within SGL_RS03010 at 246.579 kb on - strand, within SGL_RS03010 at 246.671 kb on - strand, within SGL_RS03010 at 246.722 kb on - strand, within SGL_RS03010 at 246.850 kb on - strand, within SGL_RS03010 at 247.065 kb on - strand at 247.123 kb on + strand at 247.188 kb on + strand, within SGL_RS03015 at 247.301 kb on + strand, within SGL_RS03015 at 247.371 kb on + strand, within SGL_RS03015 at 247.515 kb on - strand, within SGL_RS03015 at 247.565 kb on - strand at 247.674 kb on - strand at 247.713 kb on - strand at 247.958 kb on - strand, within SGL_RS03020
Per-strain Table
Position Strand Gene LocusTag Fraction high light; bubbling remove 244,540 - SGL_RS03000 0.69 -0.8 244,570 - SGL_RS03000 0.75 -6.1 245,163 + SGL_RS03005 0.62 -6.9 245,171 - SGL_RS03005 0.63 -6.7 245,406 - -6.9 245,426 - -5.9 245,468 - -0.0 245,601 - +0.8 245,620 - -0.7 245,779 - SGL_RS03010 0.18 -0.0 245,787 - SGL_RS03010 0.19 -0.5 245,833 + SGL_RS03010 0.22 +1.1 245,833 + SGL_RS03010 0.22 -0.1 245,841 - SGL_RS03010 0.22 +0.1 245,950 + SGL_RS03010 0.30 +0.9 246,097 + SGL_RS03010 0.39 -0.0 246,353 - SGL_RS03010 0.56 -0.1 246,353 - SGL_RS03010 0.56 -1.5 246,371 - SGL_RS03010 0.57 -0.1 246,560 - SGL_RS03010 0.70 +0.2 246,571 + SGL_RS03010 0.70 -0.5 246,579 - SGL_RS03010 0.71 -0.1 246,671 - SGL_RS03010 0.77 -0.3 246,722 - SGL_RS03010 0.80 +0.1 246,850 - SGL_RS03010 0.89 +0.4 247,065 - -0.1 247,123 + +0.1 247,188 + SGL_RS03015 0.16 +0.4 247,301 + SGL_RS03015 0.41 +0.1 247,371 + SGL_RS03015 0.56 +0.2 247,515 - SGL_RS03015 0.88 +0.8 247,565 - +0.4 247,674 - -1.6 247,713 - +1.0 247,958 - SGL_RS03020 0.28 -2.6
Or see this region's nucleotide sequence