Experiment: medium light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14240 and SGL_RS14245 are separated by 192 nucleotides SGL_RS14245 and SGL_RS14250 are separated by 442 nucleotides SGL_RS14250 and SGL_RS14255 are separated by 27 nucleotides
SGL_RS14240: SGL_RS14240 - preprotein translocase subunit SecA, at 2,647,386 to 2,650,184
_RS14240
SGL_RS14245: SGL_RS14245 - TMEM165/GDT1 family protein, at 2,650,377 to 2,650,997
_RS14245
SGL_RS14250: SGL_RS14250 - DUF4253 domain-containing protein, at 2,651,440 to 2,651,838
_RS14250
SGL_RS14255: SGL_RS14255 - Holliday junction branch migration DNA helicase RuvB, at 2,651,866 to 2,652,951
_RS14255
Position (kb)
2650
2651 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 2650.354 kb on + strand at 2650.538 kb on - strand, within SGL_RS14245 at 2650.568 kb on - strand, within SGL_RS14245 at 2650.573 kb on + strand, within SGL_RS14245 at 2650.623 kb on + strand, within SGL_RS14245 at 2650.882 kb on - strand, within SGL_RS14245 at 2651.317 kb on - strand at 2651.337 kb on - strand at 2651.455 kb on + strand at 2651.494 kb on + strand, within SGL_RS14250 at 2651.504 kb on + strand, within SGL_RS14250 at 2651.581 kb on - strand, within SGL_RS14250 at 2651.779 kb on + strand, within SGL_RS14250 at 2651.899 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction medium light; bubbling remove 2,650,354 + +0.6 2,650,538 - SGL_RS14245 0.26 -5.6 2,650,568 - SGL_RS14245 0.31 -3.0 2,650,573 + SGL_RS14245 0.32 -3.0 2,650,623 + SGL_RS14245 0.40 -4.1 2,650,882 - SGL_RS14245 0.81 -6.2 2,651,317 - -0.1 2,651,337 - +0.5 2,651,455 + +3.3 2,651,494 + SGL_RS14250 0.14 -1.9 2,651,504 + SGL_RS14250 0.16 +0.1 2,651,581 - SGL_RS14250 0.35 -0.0 2,651,779 + SGL_RS14250 0.85 -1.5 2,651,899 - -0.3
Or see this region's nucleotide sequence