Experiment: medium light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12180 and SGL_RS12185 are separated by 0 nucleotides SGL_RS12185 and SGL_RS12190 are separated by 247 nucleotides SGL_RS12190 and SGL_RS12195 are separated by 30 nucleotides SGL_RS12195 and SGL_RS12200 are separated by 111 nucleotides
SGL_RS12180: SGL_RS12180 - type II toxin-antitoxin system HicB family antitoxin, at 2,193,591 to 2,193,812
_RS12180
SGL_RS12185: SGL_RS12185 - type II toxin-antitoxin system HicA family toxin, at 2,193,813 to 2,194,157
_RS12185
SGL_RS12190: SGL_RS12190 - ATP-dependent chaperone ClpB, at 2,194,405 to 2,197,101
_RS12190
SGL_RS12195: SGL_RS12195 - hypothetical protein, at 2,197,132 to 2,197,620
_RS12195
SGL_RS12200: SGL_RS12200 - efflux RND transporter permease subunit, at 2,197,732 to 2,200,959
_RS12200
Position (kb)
2194
2195
2196
2197
2198 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2193.657 kb on + strand, within SGL_RS12180 at 2197.377 kb on + strand, within SGL_RS12195 at 2197.471 kb on + strand, within SGL_RS12195 at 2197.536 kb on + strand, within SGL_RS12195 at 2197.544 kb on - strand, within SGL_RS12195 at 2197.643 kb on - strand at 2197.905 kb on - strand at 2197.998 kb on + strand at 2198.004 kb on - strand at 2198.017 kb on - strand at 2198.070 kb on - strand, within SGL_RS12200
Per-strain Table
Position Strand Gene LocusTag Fraction medium light; bubbling remove 2,193,657 + SGL_RS12180 0.30 +1.4 2,197,377 + SGL_RS12195 0.50 -0.5 2,197,471 + SGL_RS12195 0.69 +0.9 2,197,536 + SGL_RS12195 0.83 -1.5 2,197,544 - SGL_RS12195 0.84 +0.1 2,197,643 - -1.4 2,197,905 - -0.8 2,197,998 + -0.3 2,198,004 - -3.7 2,198,017 - -0.3 2,198,070 - SGL_RS12200 0.10 -1.1
Or see this region's nucleotide sequence