Experiment: medium light; bubbling
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00245 and SGL_RS00250 are separated by 92 nucleotides SGL_RS00250 and SGL_RS00255 are separated by 0 nucleotides SGL_RS00255 and SGL_RS19680 are separated by 75 nucleotides SGL_RS19680 and SGL_RS00260 are separated by 236 nucleotides
SGL_RS00245: SGL_RS00245 - glycosyltransferase, at 53,037 to 54,026
_RS00245
SGL_RS00250: SGL_RS00250 - WecB/TagA/CpsF family glycosyltransferase, at 54,119 to 54,910
_RS00250
SGL_RS00255: SGL_RS00255 - glycosyltransferase family 2 protein, at 54,911 to 56,281
_RS00255
SGL_RS19680: SGL_RS19680 - IS1 family transposase, at 56,357 to 56,617
_RS19680
SGL_RS00260: SGL_RS00260 - sugar transferase, at 56,854 to 57,807
_RS00260
Position (kb) 54
55
56
57 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 53.971 kb on + strand at 54.068 kb on - strand at 54.209 kb on - strand, within SGL_RS00250 at 54.608 kb on - strand, within SGL_RS00250 at 54.608 kb on - strand, within SGL_RS00250 at 54.638 kb on - strand, within SGL_RS00250 at 54.686 kb on + strand, within SGL_RS00250 at 54.859 kb on + strand at 54.859 kb on + strand at 54.868 kb on + strand at 54.888 kb on - strand at 54.905 kb on + strand at 54.929 kb on + strand at 54.956 kb on + strand at 54.966 kb on + strand at 54.966 kb on + strand at 54.966 kb on + strand at 54.974 kb on - strand at 55.129 kb on + strand, within SGL_RS00255 at 55.225 kb on - strand, within SGL_RS00255 at 55.241 kb on + strand, within SGL_RS00255 at 55.249 kb on - strand, within SGL_RS00255 at 55.359 kb on + strand, within SGL_RS00255 at 55.414 kb on + strand, within SGL_RS00255 at 55.414 kb on + strand, within SGL_RS00255 at 55.432 kb on - strand, within SGL_RS00255 at 55.544 kb on + strand, within SGL_RS00255 at 55.558 kb on + strand, within SGL_RS00255 at 55.691 kb on + strand, within SGL_RS00255 at 55.721 kb on + strand, within SGL_RS00255 at 55.732 kb on + strand, within SGL_RS00255 at 55.795 kb on + strand, within SGL_RS00255 at 55.835 kb on + strand, within SGL_RS00255 at 55.843 kb on - strand, within SGL_RS00255 at 55.843 kb on - strand, within SGL_RS00255 at 55.845 kb on + strand, within SGL_RS00255 at 55.845 kb on + strand, within SGL_RS00255 at 55.853 kb on - strand, within SGL_RS00255 at 55.853 kb on - strand, within SGL_RS00255 at 56.090 kb on - strand, within SGL_RS00255 at 56.149 kb on - strand at 56.161 kb on + strand at 56.161 kb on + strand at 56.379 kb on + strand at 56.387 kb on - strand, within SGL_RS19680 at 56.557 kb on + strand, within SGL_RS19680 at 56.576 kb on - strand, within SGL_RS19680 at 56.645 kb on + strand at 56.653 kb on - strand at 56.775 kb on + strand at 56.804 kb on - strand at 57.000 kb on - strand, within SGL_RS00260 at 57.046 kb on - strand, within SGL_RS00260 at 57.058 kb on + strand, within SGL_RS00260 at 57.125 kb on + strand, within SGL_RS00260 at 57.236 kb on + strand, within SGL_RS00260
Per-strain Table
Position Strand Gene LocusTag Fraction medium light; bubbling remove 53,971 + -2.9 54,068 - -4.6 54,209 - SGL_RS00250 0.11 -2.5 54,608 - SGL_RS00250 0.62 -2.7 54,608 - SGL_RS00250 0.62 -3.0 54,638 - SGL_RS00250 0.66 -0.9 54,686 + SGL_RS00250 0.72 -3.6 54,859 + -0.2 54,859 + +0.5 54,868 + -2.5 54,888 - +0.5 54,905 + -0.2 54,929 + -0.1 54,956 + +3.0 54,966 + -3.4 54,966 + -0.5 54,966 + +0.2 54,974 - -0.1 55,129 + SGL_RS00255 0.16 -0.6 55,225 - SGL_RS00255 0.23 -4.2 55,241 + SGL_RS00255 0.24 -3.8 55,249 - SGL_RS00255 0.25 -4.3 55,359 + SGL_RS00255 0.33 -3.5 55,414 + SGL_RS00255 0.37 -1.7 55,414 + SGL_RS00255 0.37 -4.8 55,432 - SGL_RS00255 0.38 -4.4 55,544 + SGL_RS00255 0.46 -4.9 55,558 + SGL_RS00255 0.47 -4.9 55,691 + SGL_RS00255 0.57 -6.4 55,721 + SGL_RS00255 0.59 -4.0 55,732 + SGL_RS00255 0.60 -3.7 55,795 + SGL_RS00255 0.64 -3.9 55,835 + SGL_RS00255 0.67 -1.7 55,843 - SGL_RS00255 0.68 -4.7 55,843 - SGL_RS00255 0.68 -1.5 55,845 + SGL_RS00255 0.68 -6.1 55,845 + SGL_RS00255 0.68 -4.3 55,853 - SGL_RS00255 0.69 -3.8 55,853 - SGL_RS00255 0.69 -2.6 56,090 - SGL_RS00255 0.86 -5.6 56,149 - -4.2 56,161 + -1.3 56,161 + -2.8 56,379 + +0.7 56,387 - SGL_RS19680 0.11 +0.4 56,557 + SGL_RS19680 0.77 -0.0 56,576 - SGL_RS19680 0.84 +0.0 56,645 + -0.4 56,653 - +0.2 56,775 + -0.7 56,804 - -0.7 57,000 - SGL_RS00260 0.15 +1.1 57,046 - SGL_RS00260 0.20 +0.4 57,058 + SGL_RS00260 0.21 +0.4 57,125 + SGL_RS00260 0.28 -0.9 57,236 + SGL_RS00260 0.40 -0.6
Or see this region's nucleotide sequence