Experiment: Zinc 15 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10465 and SGL_RS10470 are separated by 235 nucleotides SGL_RS10470 and SGL_RS10475 are separated by 130 nucleotides SGL_RS10475 and SGL_RS10480 are separated by 281 nucleotides SGL_RS10480 and SGL_RS10485 are separated by 220 nucleotides SGL_RS10485 and SGL_RS10490 are separated by 131 nucleotides
SGL_RS10465: SGL_RS10465 - universal stress protein, at 1,822,666 to 1,823,130
_RS10465
SGL_RS10470: SGL_RS10470 - tRNA-Arg, at 1,823,366 to 1,823,439
_RS10470
SGL_RS10475: SGL_RS10475 - cytochrome b6-f complex subunit PetG, at 1,823,570 to 1,823,686
_RS10475
SGL_RS10480: SGL_RS10480 - metallophosphoesterase family protein, at 1,823,968 to 1,824,732
_RS10480
SGL_RS10485: SGL_RS10485 - ComEA family DNA-binding protein, at 1,824,953 to 1,825,468
_RS10485
SGL_RS10490: SGL_RS10490 - precorrin-8X methylmutase, at 1,825,600 to 1,826,226
_RS10490
Position (kb)
1823
1824
1825 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1823.023 kb on - strand, within SGL_RS10465 at 1823.097 kb on + strand at 1823.265 kb on - strand at 1824.016 kb on + strand at 1824.036 kb on + strand at 1824.079 kb on + strand, within SGL_RS10480 at 1824.188 kb on + strand, within SGL_RS10480 at 1824.214 kb on + strand, within SGL_RS10480 at 1824.239 kb on - strand, within SGL_RS10480 at 1824.349 kb on + strand, within SGL_RS10480 at 1824.349 kb on + strand, within SGL_RS10480 at 1824.352 kb on - strand, within SGL_RS10480 at 1824.357 kb on - strand, within SGL_RS10480 at 1824.380 kb on + strand, within SGL_RS10480 at 1824.383 kb on - strand, within SGL_RS10480 at 1824.415 kb on + strand, within SGL_RS10480 at 1824.517 kb on + strand, within SGL_RS10480 at 1824.627 kb on + strand, within SGL_RS10480 at 1824.772 kb on + strand at 1824.857 kb on + strand at 1824.868 kb on + strand at 1824.876 kb on - strand at 1824.902 kb on + strand at 1825.418 kb on - strand at 1825.665 kb on + strand, within SGL_RS10490 at 1825.671 kb on - strand, within SGL_RS10490 at 1825.678 kb on - strand, within SGL_RS10490 at 1825.680 kb on + strand, within SGL_RS10490 at 1825.680 kb on + strand, within SGL_RS10490 at 1825.688 kb on - strand, within SGL_RS10490
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc 15 uM remove 1,823,023 - SGL_RS10465 0.77 -0.3 1,823,097 + -0.0 1,823,265 - -0.1 1,824,016 + +0.1 1,824,036 + -0.8 1,824,079 + SGL_RS10480 0.15 -2.7 1,824,188 + SGL_RS10480 0.29 -3.0 1,824,214 + SGL_RS10480 0.32 -1.9 1,824,239 - SGL_RS10480 0.35 -1.8 1,824,349 + SGL_RS10480 0.50 -1.9 1,824,349 + SGL_RS10480 0.50 -1.6 1,824,352 - SGL_RS10480 0.50 -2.7 1,824,357 - SGL_RS10480 0.51 -1.9 1,824,380 + SGL_RS10480 0.54 -2.9 1,824,383 - SGL_RS10480 0.54 -2.4 1,824,415 + SGL_RS10480 0.58 -2.3 1,824,517 + SGL_RS10480 0.72 -2.8 1,824,627 + SGL_RS10480 0.86 -2.3 1,824,772 + -0.2 1,824,857 + -0.2 1,824,868 + -0.2 1,824,876 - +0.2 1,824,902 + -0.1 1,825,418 - -0.9 1,825,665 + SGL_RS10490 0.10 +0.7 1,825,671 - SGL_RS10490 0.11 -0.7 1,825,678 - SGL_RS10490 0.12 +0.6 1,825,680 + SGL_RS10490 0.13 -0.2 1,825,680 + SGL_RS10490 0.13 -1.8 1,825,688 - SGL_RS10490 0.14 -0.3
Or see this region's nucleotide sequence