Experiment: Zinc 15 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09835 and SGL_RS09840 overlap by 1 nucleotides SGL_RS09840 and SGL_RS09845 are separated by 67 nucleotides SGL_RS09845 and SGL_RS09850 are separated by 7 nucleotides
SGL_RS09835: SGL_RS09835 - 16S rRNA (cytidine(1402)-2'-O)-methyltransferase, at 1,691,493 to 1,692,332
_RS09835
SGL_RS09840: SGL_RS09840 - hypothetical protein, at 1,692,332 to 1,692,559
_RS09840
SGL_RS09845: SGL_RS09845 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 1,692,627 to 1,693,538
_RS09845
SGL_RS09850: SGL_RS09850 - Gfo/Idh/MocA family oxidoreductase, at 1,693,546 to 1,694,661
_RS09850
Position (kb)
1692
1693
1694 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1691.650 kb on - strand, within SGL_RS09835 at 1691.793 kb on - strand, within SGL_RS09835 at 1691.886 kb on - strand, within SGL_RS09835 at 1691.963 kb on + strand, within SGL_RS09835 at 1691.971 kb on - strand, within SGL_RS09835 at 1691.985 kb on + strand, within SGL_RS09835 at 1692.025 kb on - strand, within SGL_RS09835 at 1692.051 kb on + strand, within SGL_RS09835 at 1692.059 kb on - strand, within SGL_RS09835 at 1692.140 kb on + strand, within SGL_RS09835 at 1692.140 kb on + strand, within SGL_RS09835 at 1692.373 kb on + strand, within SGL_RS09840 at 1692.373 kb on + strand, within SGL_RS09840 at 1692.413 kb on + strand, within SGL_RS09840 at 1692.464 kb on + strand, within SGL_RS09840 at 1692.472 kb on - strand, within SGL_RS09840 at 1692.551 kb on - strand at 1692.780 kb on - strand, within SGL_RS09845 at 1692.842 kb on + strand, within SGL_RS09845 at 1692.844 kb on + strand, within SGL_RS09845 at 1692.852 kb on - strand, within SGL_RS09845 at 1692.865 kb on + strand, within SGL_RS09845 at 1692.865 kb on + strand, within SGL_RS09845 at 1692.873 kb on - strand, within SGL_RS09845 at 1693.077 kb on - strand, within SGL_RS09845 at 1693.149 kb on + strand, within SGL_RS09845 at 1693.185 kb on + strand, within SGL_RS09845 at 1693.194 kb on - strand, within SGL_RS09845 at 1693.230 kb on + strand, within SGL_RS09845 at 1693.298 kb on - strand, within SGL_RS09845 at 1693.578 kb on + strand at 1693.583 kb on + strand at 1693.684 kb on + strand, within SGL_RS09850 at 1693.704 kb on - strand, within SGL_RS09850 at 1693.825 kb on + strand, within SGL_RS09850 at 1693.971 kb on - strand, within SGL_RS09850 at 1693.971 kb on - strand, within SGL_RS09850 at 1694.015 kb on + strand, within SGL_RS09850 at 1694.015 kb on + strand, within SGL_RS09850 at 1694.023 kb on - strand, within SGL_RS09850 at 1694.023 kb on - strand, within SGL_RS09850 at 1694.062 kb on - strand, within SGL_RS09850 at 1694.067 kb on - strand, within SGL_RS09850 at 1694.098 kb on + strand, within SGL_RS09850 at 1694.105 kb on - strand, within SGL_RS09850 at 1694.158 kb on - strand, within SGL_RS09850 at 1694.209 kb on - strand, within SGL_RS09850 at 1694.267 kb on - strand, within SGL_RS09850 at 1694.312 kb on - strand, within SGL_RS09850 at 1694.331 kb on + strand, within SGL_RS09850 at 1694.524 kb on + strand, within SGL_RS09850 at 1694.524 kb on + strand, within SGL_RS09850 at 1694.532 kb on - strand, within SGL_RS09850
Per-strain Table
Position Strand Gene LocusTag Fraction Zinc 15 uM remove 1,691,650 - SGL_RS09835 0.19 +0.1 1,691,793 - SGL_RS09835 0.36 +0.2 1,691,886 - SGL_RS09835 0.47 +0.5 1,691,963 + SGL_RS09835 0.56 +0.4 1,691,971 - SGL_RS09835 0.57 +0.0 1,691,985 + SGL_RS09835 0.59 -0.2 1,692,025 - SGL_RS09835 0.63 -0.1 1,692,051 + SGL_RS09835 0.66 +0.9 1,692,059 - SGL_RS09835 0.67 -1.4 1,692,140 + SGL_RS09835 0.77 -0.2 1,692,140 + SGL_RS09835 0.77 -0.4 1,692,373 + SGL_RS09840 0.18 +0.2 1,692,373 + SGL_RS09840 0.18 -0.7 1,692,413 + SGL_RS09840 0.36 +0.3 1,692,464 + SGL_RS09840 0.58 +0.7 1,692,472 - SGL_RS09840 0.61 +0.0 1,692,551 - +0.1 1,692,780 - SGL_RS09845 0.17 -4.2 1,692,842 + SGL_RS09845 0.24 -2.4 1,692,844 + SGL_RS09845 0.24 -2.0 1,692,852 - SGL_RS09845 0.25 -5.2 1,692,865 + SGL_RS09845 0.26 -4.7 1,692,865 + SGL_RS09845 0.26 -2.7 1,692,873 - SGL_RS09845 0.27 -4.2 1,693,077 - SGL_RS09845 0.49 -3.3 1,693,149 + SGL_RS09845 0.57 -2.1 1,693,185 + SGL_RS09845 0.61 -4.5 1,693,194 - SGL_RS09845 0.62 -2.7 1,693,230 + SGL_RS09845 0.66 -3.7 1,693,298 - SGL_RS09845 0.74 -1.9 1,693,578 + +1.1 1,693,583 + -0.5 1,693,684 + SGL_RS09850 0.12 -0.1 1,693,704 - SGL_RS09850 0.14 +0.2 1,693,825 + SGL_RS09850 0.25 -0.0 1,693,971 - SGL_RS09850 0.38 -1.1 1,693,971 - SGL_RS09850 0.38 -0.8 1,694,015 + SGL_RS09850 0.42 +0.5 1,694,015 + SGL_RS09850 0.42 -0.6 1,694,023 - SGL_RS09850 0.43 -0.1 1,694,023 - SGL_RS09850 0.43 -0.3 1,694,062 - SGL_RS09850 0.46 +0.1 1,694,067 - SGL_RS09850 0.47 -0.4 1,694,098 + SGL_RS09850 0.49 -0.2 1,694,105 - SGL_RS09850 0.50 -1.1 1,694,158 - SGL_RS09850 0.55 +0.0 1,694,209 - SGL_RS09850 0.59 -0.4 1,694,267 - SGL_RS09850 0.65 -0.1 1,694,312 - SGL_RS09850 0.69 -0.1 1,694,331 + SGL_RS09850 0.70 +0.3 1,694,524 + SGL_RS09850 0.88 -0.6 1,694,524 + SGL_RS09850 0.88 -0.3 1,694,532 - SGL_RS09850 0.88 -0.5
Or see this region's nucleotide sequence