Experiment: Manganese (II) chloride tetrahydrate 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15685 and SGL_RS15695 are separated by 488 nucleotides SGL_RS15695 and SGL_RS15700 are separated by 274 nucleotides
SGL_RS15685: SGL_RS15685 - IS701 family transposase, at 2,968,175 to 2,969,188
_RS15685
SGL_RS15695: SGL_RS15695 - ammonium transporter, at 2,969,677 to 2,971,200
_RS15695
SGL_RS15700: SGL_RS15700 - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 2,971,475 to 2,972,167
_RS15700
Position (kb)
2969
2970
2971
2972 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2969.443 kb on + strand at 2969.494 kb on - strand at 2969.749 kb on - strand at 2969.808 kb on - strand at 2969.810 kb on + strand at 2969.856 kb on - strand, within SGL_RS15695 at 2969.896 kb on + strand, within SGL_RS15695 at 2969.963 kb on - strand, within SGL_RS15695 at 2969.988 kb on + strand, within SGL_RS15695 at 2970.044 kb on - strand, within SGL_RS15695 at 2970.141 kb on + strand, within SGL_RS15695 at 2970.144 kb on - strand, within SGL_RS15695 at 2970.200 kb on - strand, within SGL_RS15695 at 2970.201 kb on - strand, within SGL_RS15695 at 2970.239 kb on - strand, within SGL_RS15695 at 2970.393 kb on + strand, within SGL_RS15695 at 2970.485 kb on + strand, within SGL_RS15695 at 2970.534 kb on + strand, within SGL_RS15695 at 2970.555 kb on - strand, within SGL_RS15695 at 2970.652 kb on + strand, within SGL_RS15695 at 2970.693 kb on - strand, within SGL_RS15695 at 2970.771 kb on - strand, within SGL_RS15695 at 2970.876 kb on - strand, within SGL_RS15695 at 2970.932 kb on + strand, within SGL_RS15695 at 2970.933 kb on + strand, within SGL_RS15695 at 2970.933 kb on + strand, within SGL_RS15695 at 2970.940 kb on - strand, within SGL_RS15695 at 2971.582 kb on + strand, within SGL_RS15700 at 2971.631 kb on + strand, within SGL_RS15700 at 2971.643 kb on + strand, within SGL_RS15700 at 2971.676 kb on + strand, within SGL_RS15700 at 2971.683 kb on + strand, within SGL_RS15700 at 2971.701 kb on + strand, within SGL_RS15700 at 2971.811 kb on - strand, within SGL_RS15700 at 2971.839 kb on + strand, within SGL_RS15700 at 2971.917 kb on - strand, within SGL_RS15700 at 2971.977 kb on - strand, within SGL_RS15700 at 2972.008 kb on - strand, within SGL_RS15700 at 2972.046 kb on - strand, within SGL_RS15700 at 2972.114 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 150 uM remove 2,969,443 + +0.3 2,969,494 - +0.1 2,969,749 - +0.2 2,969,808 - -0.8 2,969,810 + -1.4 2,969,856 - SGL_RS15695 0.12 -1.0 2,969,896 + SGL_RS15695 0.14 -0.6 2,969,963 - SGL_RS15695 0.19 -1.9 2,969,988 + SGL_RS15695 0.20 -1.3 2,970,044 - SGL_RS15695 0.24 -1.3 2,970,141 + SGL_RS15695 0.30 -2.0 2,970,144 - SGL_RS15695 0.31 -0.9 2,970,200 - SGL_RS15695 0.34 -0.9 2,970,201 - SGL_RS15695 0.34 -0.3 2,970,239 - SGL_RS15695 0.37 -2.0 2,970,393 + SGL_RS15695 0.47 -1.0 2,970,485 + SGL_RS15695 0.53 -0.6 2,970,534 + SGL_RS15695 0.56 -1.3 2,970,555 - SGL_RS15695 0.58 -1.0 2,970,652 + SGL_RS15695 0.64 +1.1 2,970,693 - SGL_RS15695 0.67 -0.6 2,970,771 - SGL_RS15695 0.72 -1.1 2,970,876 - SGL_RS15695 0.79 -2.1 2,970,932 + SGL_RS15695 0.82 -2.9 2,970,933 + SGL_RS15695 0.82 -2.0 2,970,933 + SGL_RS15695 0.82 -0.2 2,970,940 - SGL_RS15695 0.83 -1.2 2,971,582 + SGL_RS15700 0.15 -3.2 2,971,631 + SGL_RS15700 0.23 -2.5 2,971,643 + SGL_RS15700 0.24 -3.5 2,971,676 + SGL_RS15700 0.29 -3.1 2,971,683 + SGL_RS15700 0.30 -2.7 2,971,701 + SGL_RS15700 0.33 -4.0 2,971,811 - SGL_RS15700 0.48 -4.6 2,971,839 + SGL_RS15700 0.53 -2.3 2,971,917 - SGL_RS15700 0.64 -3.2 2,971,977 - SGL_RS15700 0.72 -2.9 2,972,008 - SGL_RS15700 0.77 -3.6 2,972,046 - SGL_RS15700 0.82 -2.2 2,972,114 - -0.5
Or see this region's nucleotide sequence