Experiment: Manganese (II) chloride tetrahydrate 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14295 and SGL_RS14300 are separated by 214 nucleotides SGL_RS14300 and SGL_RS14305 are separated by 81 nucleotides SGL_RS14305 and SGL_RS14310 overlap by 46 nucleotides
SGL_RS14295: SGL_RS14295 - mechanosensitive ion channel, at 2,660,854 to 2,661,744
_RS14295
SGL_RS14300: SGL_RS14300 - HAMP domain-containing sensor histidine kinase, at 2,661,959 to 2,663,284
_RS14300
SGL_RS14305: SGL_RS14305 - DUF3593 domain-containing protein, at 2,663,366 to 2,663,731
_RS14305
SGL_RS14310: SGL_RS14310 - DUF2499 domain-containing protein, at 2,663,686 to 2,664,000
_RS14310
Position (kb)
2661
2662
2663
2664 Strain fitness (log2 ratio)
-8
-7
-6
-5
-4
-3
-2
-1
0
1 at 2661.969 kb on - strand at 2661.981 kb on - strand at 2662.094 kb on - strand, within SGL_RS14300 at 2662.106 kb on - strand, within SGL_RS14300 at 2662.141 kb on - strand, within SGL_RS14300 at 2662.181 kb on - strand, within SGL_RS14300 at 2662.302 kb on - strand, within SGL_RS14300 at 2662.373 kb on + strand, within SGL_RS14300 at 2662.373 kb on + strand, within SGL_RS14300 at 2662.381 kb on - strand, within SGL_RS14300 at 2662.381 kb on - strand, within SGL_RS14300 at 2662.381 kb on - strand, within SGL_RS14300 at 2662.627 kb on - strand, within SGL_RS14300 at 2662.644 kb on + strand, within SGL_RS14300 at 2662.652 kb on - strand, within SGL_RS14300 at 2662.657 kb on - strand, within SGL_RS14300 at 2662.680 kb on + strand, within SGL_RS14300 at 2662.734 kb on - strand, within SGL_RS14300 at 2662.947 kb on + strand, within SGL_RS14300 at 2662.954 kb on - strand, within SGL_RS14300 at 2662.974 kb on + strand, within SGL_RS14300 at 2662.994 kb on - strand, within SGL_RS14300 at 2662.998 kb on + strand, within SGL_RS14300 at 2663.005 kb on + strand, within SGL_RS14300 at 2663.008 kb on + strand, within SGL_RS14300 at 2663.028 kb on - strand, within SGL_RS14300 at 2663.081 kb on + strand, within SGL_RS14300 at 2663.091 kb on - strand, within SGL_RS14300 at 2663.195 kb on - strand at 2663.239 kb on - strand at 2663.320 kb on + strand at 2663.473 kb on - strand, within SGL_RS14305 at 2663.522 kb on - strand, within SGL_RS14305 at 2663.770 kb on + strand, within SGL_RS14310 at 2663.770 kb on + strand, within SGL_RS14310 at 2663.784 kb on - strand, within SGL_RS14310 at 2663.787 kb on + strand, within SGL_RS14310 at 2663.844 kb on - strand, within SGL_RS14310 at 2663.870 kb on + strand, within SGL_RS14310 at 2663.875 kb on + strand, within SGL_RS14310 at 2663.875 kb on + strand, within SGL_RS14310 at 2663.875 kb on + strand, within SGL_RS14310 at 2663.875 kb on + strand, within SGL_RS14310 at 2663.878 kb on - strand, within SGL_RS14310 at 2663.878 kb on - strand, within SGL_RS14310 at 2663.893 kb on + strand, within SGL_RS14310 at 2663.901 kb on - strand, within SGL_RS14310 at 2663.901 kb on - strand, within SGL_RS14310 at 2663.909 kb on + strand, within SGL_RS14310 at 2663.921 kb on - strand, within SGL_RS14310 at 2664.068 kb on + strand at 2664.076 kb on - strand at 2664.111 kb on + strand at 2664.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 150 uM remove 2,661,969 - -4.8 2,661,981 - -4.7 2,662,094 - SGL_RS14300 0.10 -7.7 2,662,106 - SGL_RS14300 0.11 -6.7 2,662,141 - SGL_RS14300 0.14 -6.6 2,662,181 - SGL_RS14300 0.17 -6.7 2,662,302 - SGL_RS14300 0.26 -6.5 2,662,373 + SGL_RS14300 0.31 -7.7 2,662,373 + SGL_RS14300 0.31 -3.8 2,662,381 - SGL_RS14300 0.32 -6.8 2,662,381 - SGL_RS14300 0.32 -3.6 2,662,381 - SGL_RS14300 0.32 -4.1 2,662,627 - SGL_RS14300 0.50 -7.1 2,662,644 + SGL_RS14300 0.52 -6.1 2,662,652 - SGL_RS14300 0.52 -5.4 2,662,657 - SGL_RS14300 0.53 -3.8 2,662,680 + SGL_RS14300 0.54 -6.7 2,662,734 - SGL_RS14300 0.58 -7.8 2,662,947 + SGL_RS14300 0.75 -6.2 2,662,954 - SGL_RS14300 0.75 -2.8 2,662,974 + SGL_RS14300 0.77 -4.8 2,662,994 - SGL_RS14300 0.78 -8.5 2,662,998 + SGL_RS14300 0.78 -6.9 2,663,005 + SGL_RS14300 0.79 -7.6 2,663,008 + SGL_RS14300 0.79 -6.5 2,663,028 - SGL_RS14300 0.81 -8.4 2,663,081 + SGL_RS14300 0.85 -6.9 2,663,091 - SGL_RS14300 0.85 -6.9 2,663,195 - -7.8 2,663,239 - -7.0 2,663,320 + +0.1 2,663,473 - SGL_RS14305 0.29 -0.0 2,663,522 - SGL_RS14305 0.43 -0.1 2,663,770 + SGL_RS14310 0.27 -0.4 2,663,770 + SGL_RS14310 0.27 +0.1 2,663,784 - SGL_RS14310 0.31 -0.1 2,663,787 + SGL_RS14310 0.32 -0.2 2,663,844 - SGL_RS14310 0.50 +0.0 2,663,870 + SGL_RS14310 0.58 -0.0 2,663,875 + SGL_RS14310 0.60 -0.2 2,663,875 + SGL_RS14310 0.60 -0.1 2,663,875 + SGL_RS14310 0.60 -0.2 2,663,875 + SGL_RS14310 0.60 +0.1 2,663,878 - SGL_RS14310 0.61 -0.4 2,663,878 - SGL_RS14310 0.61 +0.5 2,663,893 + SGL_RS14310 0.66 +0.1 2,663,901 - SGL_RS14310 0.68 -0.1 2,663,901 - SGL_RS14310 0.68 +0.0 2,663,909 + SGL_RS14310 0.71 +0.5 2,663,921 - SGL_RS14310 0.75 +0.5 2,664,068 + +0.0 2,664,076 - +0.3 2,664,111 + -0.4 2,664,249 - -0.5
Or see this region's nucleotide sequence