Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS14245

Experiment: Manganese (II) chloride tetrahydrate 150 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS14240 and SGL_RS14245 are separated by 192 nucleotidesSGL_RS14245 and SGL_RS14250 are separated by 442 nucleotidesSGL_RS14250 and SGL_RS14255 are separated by 27 nucleotides SGL_RS14240: SGL_RS14240 - preprotein translocase subunit SecA, at 2,647,386 to 2,650,184 _RS14240 SGL_RS14245: SGL_RS14245 - TMEM165/GDT1 family protein, at 2,650,377 to 2,650,997 _RS14245 SGL_RS14250: SGL_RS14250 - DUF4253 domain-containing protein, at 2,651,440 to 2,651,838 _RS14250 SGL_RS14255: SGL_RS14255 - Holliday junction branch migration DNA helicase RuvB, at 2,651,866 to 2,652,951 _RS14255 Position (kb) 2650 2651Strain fitness (log2 ratio) -8 -7 -6 -5 -4 -3 -2 -1 0 1at 2650.354 kb on + strandat 2650.538 kb on - strand, within SGL_RS14245at 2650.568 kb on - strand, within SGL_RS14245at 2650.573 kb on + strand, within SGL_RS14245at 2650.623 kb on + strand, within SGL_RS14245at 2650.882 kb on - strand, within SGL_RS14245at 2651.317 kb on - strandat 2651.337 kb on - strandat 2651.455 kb on + strandat 2651.494 kb on + strand, within SGL_RS14250at 2651.504 kb on + strand, within SGL_RS14250at 2651.581 kb on - strand, within SGL_RS14250at 2651.779 kb on + strand, within SGL_RS14250at 2651.899 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 150 uM
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2,650,354 + +0.3
2,650,538 - SGL_RS14245 0.26 -8.7
2,650,568 - SGL_RS14245 0.31 -7.1
2,650,573 + SGL_RS14245 0.32 -6.0
2,650,623 + SGL_RS14245 0.40 -4.9
2,650,882 - SGL_RS14245 0.81 -7.3
2,651,317 - -0.2
2,651,337 - +0.2
2,651,455 + -0.0
2,651,494 + SGL_RS14250 0.14 +0.5
2,651,504 + SGL_RS14250 0.16 -0.1
2,651,581 - SGL_RS14250 0.35 +0.2
2,651,779 + SGL_RS14250 0.85 +0.1
2,651,899 - +0.1

Or see this region's nucleotide sequence