Experiment: Manganese (II) chloride tetrahydrate 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12475 and SGL_RS12480 are separated by 242 nucleotides SGL_RS12480 and SGL_RS12485 are separated by 32 nucleotides SGL_RS12485 and SGL_RS12490 are separated by 109 nucleotides
SGL_RS12475: SGL_RS12475 - cation-translocating P-type ATPase, at 2,254,112 to 2,256,454
_RS12475
SGL_RS12480: SGL_RS12480 - FHA domain-containing protein, at 2,256,697 to 2,257,170
_RS12480
SGL_RS12485: SGL_RS12485 - YebC/PmpR family DNA-binding transcriptional regulator, at 2,257,203 to 2,257,964
_RS12485
SGL_RS12490: SGL_RS12490 - glutamate--cysteine ligase, at 2,258,074 to 2,259,243
_RS12490
Position (kb)
2257
2258 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2256.219 kb on + strand, within SGL_RS12475 at 2256.245 kb on - strand at 2256.406 kb on + strand at 2256.777 kb on + strand, within SGL_RS12480 at 2256.933 kb on - strand, within SGL_RS12480 at 2257.053 kb on + strand, within SGL_RS12480 at 2257.063 kb on + strand, within SGL_RS12480 at 2257.233 kb on - strand at 2257.316 kb on - strand, within SGL_RS12485 at 2257.321 kb on - strand, within SGL_RS12485 at 2257.426 kb on + strand, within SGL_RS12485 at 2257.434 kb on - strand, within SGL_RS12485 at 2257.470 kb on + strand, within SGL_RS12485 at 2257.478 kb on - strand, within SGL_RS12485 at 2257.494 kb on + strand, within SGL_RS12485 at 2257.494 kb on - strand, within SGL_RS12485 at 2257.494 kb on - strand, within SGL_RS12485 at 2258.026 kb on + strand at 2258.215 kb on - strand, within SGL_RS12490 at 2258.309 kb on - strand, within SGL_RS12490 at 2258.332 kb on + strand, within SGL_RS12490 at 2258.340 kb on - strand, within SGL_RS12490 at 2258.393 kb on - strand, within SGL_RS12490 at 2258.439 kb on + strand, within SGL_RS12490 at 2258.550 kb on + strand, within SGL_RS12490 at 2258.856 kb on + strand, within SGL_RS12490 at 2258.937 kb on - strand, within SGL_RS12490 at 2258.937 kb on - strand, within SGL_RS12490 at 2258.942 kb on - strand, within SGL_RS12490
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 150 uM remove 2,256,219 + SGL_RS12475 0.90 +0.2 2,256,245 - -0.2 2,256,406 + +0.8 2,256,777 + SGL_RS12480 0.17 -0.5 2,256,933 - SGL_RS12480 0.50 -0.3 2,257,053 + SGL_RS12480 0.75 -1.2 2,257,063 + SGL_RS12480 0.77 -0.6 2,257,233 - -0.7 2,257,316 - SGL_RS12485 0.15 -0.9 2,257,321 - SGL_RS12485 0.15 -1.0 2,257,426 + SGL_RS12485 0.29 -0.6 2,257,434 - SGL_RS12485 0.30 -0.8 2,257,470 + SGL_RS12485 0.35 -1.5 2,257,478 - SGL_RS12485 0.36 -1.0 2,257,494 + SGL_RS12485 0.38 -1.3 2,257,494 - SGL_RS12485 0.38 -1.0 2,257,494 - SGL_RS12485 0.38 -1.0 2,258,026 + +0.2 2,258,215 - SGL_RS12490 0.12 -2.6 2,258,309 - SGL_RS12490 0.20 -2.1 2,258,332 + SGL_RS12490 0.22 -1.8 2,258,340 - SGL_RS12490 0.23 -3.8 2,258,393 - SGL_RS12490 0.27 -4.5 2,258,439 + SGL_RS12490 0.31 -2.7 2,258,550 + SGL_RS12490 0.41 -2.3 2,258,856 + SGL_RS12490 0.67 -3.3 2,258,937 - SGL_RS12490 0.74 -3.7 2,258,937 - SGL_RS12490 0.74 -5.3 2,258,942 - SGL_RS12490 0.74 -3.0
Or see this region's nucleotide sequence