Experiment: Manganese (II) chloride tetrahydrate 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10825 and SGL_RS10830 are separated by 269 nucleotides SGL_RS10830 and SGL_RS10835 are separated by 56 nucleotides SGL_RS10835 and SGL_RS10840 are separated by 24 nucleotides SGL_RS10840 and SGL_RS10845 overlap by 8 nucleotides
SGL_RS10825: SGL_RS10825 - fatty acid desaturase, at 1,895,520 to 1,896,599
_RS10825
SGL_RS10830: SGL_RS10830 - pyridoxamine 5'-phosphate oxidase, at 1,896,869 to 1,897,513
_RS10830
SGL_RS10835: SGL_RS10835 - segregation/condensation protein A, at 1,897,570 to 1,898,412
_RS10835
SGL_RS10840: SGL_RS10840 - hypothetical protein, at 1,898,437 to 1,899,240
_RS10840
SGL_RS10845: SGL_RS10845 - YgiT-type zinc finger protein, at 1,899,233 to 1,899,481
_RS10845
Position (kb) 1897
1898
1899 Strain fitness (log2 ratio) -1
0
1 at 1896.874 kb on + strand at 1896.880 kb on + strand at 1896.990 kb on + strand, within SGL_RS10830 at 1897.042 kb on + strand, within SGL_RS10830 at 1897.228 kb on - strand, within SGL_RS10830 at 1897.235 kb on + strand, within SGL_RS10830 at 1897.264 kb on - strand, within SGL_RS10830 at 1897.268 kb on + strand, within SGL_RS10830 at 1897.276 kb on - strand, within SGL_RS10830 at 1897.276 kb on - strand, within SGL_RS10830 at 1897.276 kb on - strand, within SGL_RS10830 at 1897.276 kb on - strand, within SGL_RS10830 at 1897.276 kb on - strand, within SGL_RS10830 at 1897.276 kb on - strand, within SGL_RS10830 at 1897.276 kb on - strand, within SGL_RS10830 at 1897.352 kb on - strand, within SGL_RS10830 at 1897.352 kb on - strand, within SGL_RS10830 at 1897.352 kb on - strand, within SGL_RS10830 at 1897.379 kb on - strand, within SGL_RS10830 at 1897.403 kb on - strand, within SGL_RS10830 at 1897.439 kb on - strand, within SGL_RS10830 at 1897.479 kb on - strand at 1897.683 kb on + strand, within SGL_RS10835 at 1897.747 kb on - strand, within SGL_RS10835 at 1898.407 kb on + strand at 1898.728 kb on + strand, within SGL_RS10840 at 1898.728 kb on + strand, within SGL_RS10840 at 1899.149 kb on - strand, within SGL_RS10840 at 1899.320 kb on + strand, within SGL_RS10845
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 150 uM remove 1,896,874 + -1.4 1,896,880 + +0.8 1,896,990 + SGL_RS10830 0.19 -0.8 1,897,042 + SGL_RS10830 0.27 -0.6 1,897,228 - SGL_RS10830 0.56 -0.6 1,897,235 + SGL_RS10830 0.57 -0.9 1,897,264 - SGL_RS10830 0.61 -0.8 1,897,268 + SGL_RS10830 0.62 -0.3 1,897,276 - SGL_RS10830 0.63 -0.2 1,897,276 - SGL_RS10830 0.63 -0.1 1,897,276 - SGL_RS10830 0.63 -0.1 1,897,276 - SGL_RS10830 0.63 -0.3 1,897,276 - SGL_RS10830 0.63 -0.1 1,897,276 - SGL_RS10830 0.63 -0.5 1,897,276 - SGL_RS10830 0.63 +0.3 1,897,352 - SGL_RS10830 0.75 -0.3 1,897,352 - SGL_RS10830 0.75 -0.6 1,897,352 - SGL_RS10830 0.75 -0.6 1,897,379 - SGL_RS10830 0.79 -0.1 1,897,403 - SGL_RS10830 0.83 +1.4 1,897,439 - SGL_RS10830 0.88 -0.8 1,897,479 - -0.0 1,897,683 + SGL_RS10835 0.13 -0.2 1,897,747 - SGL_RS10835 0.21 -1.6 1,898,407 + +0.1 1,898,728 + SGL_RS10840 0.36 +0.6 1,898,728 + SGL_RS10840 0.36 -0.1 1,899,149 - SGL_RS10840 0.89 -0.2 1,899,320 + SGL_RS10845 0.35 -0.2
Or see this region's nucleotide sequence