Experiment: Manganese (II) chloride tetrahydrate 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08150 and SGL_RS08155 are separated by 8 nucleotides SGL_RS08155 and SGL_RS08160 are separated by 56 nucleotides SGL_RS08160 and SGL_RS08165 overlap by 11 nucleotides SGL_RS08165 and SGL_RS08170 are separated by 114 nucleotides
SGL_RS08150: SGL_RS08150 - deoxyribodipyrimidine photo-lyase, 8-HDF type, at 1,339,656 to 1,341,122
_RS08150
SGL_RS08155: SGL_RS08155 - Spy/CpxP family protein refolding chaperone, at 1,341,131 to 1,341,637
_RS08155
SGL_RS08160: SGL_RS08160 - hypothetical protein, at 1,341,694 to 1,342,062
_RS08160
SGL_RS08165: SGL_RS08165 - sigma-70 family RNA polymerase sigma factor, at 1,342,052 to 1,342,660
_RS08165
SGL_RS08170: SGL_RS08170 - H(+)/Cl(-) exchange transporter ClcA, at 1,342,775 to 1,344,130
_RS08170
Position (kb)
1341
1342
1343 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1340.765 kb on - strand, within SGL_RS08150 at 1340.815 kb on + strand, within SGL_RS08150 at 1340.828 kb on - strand, within SGL_RS08150 at 1340.870 kb on - strand, within SGL_RS08150 at 1340.920 kb on + strand, within SGL_RS08150 at 1341.246 kb on - strand, within SGL_RS08155 at 1341.480 kb on + strand, within SGL_RS08155 at 1341.524 kb on + strand, within SGL_RS08155 at 1341.735 kb on - strand, within SGL_RS08160 at 1341.852 kb on + strand, within SGL_RS08160 at 1341.876 kb on - strand, within SGL_RS08160 at 1341.882 kb on - strand, within SGL_RS08160 at 1341.916 kb on + strand, within SGL_RS08160 at 1341.998 kb on - strand, within SGL_RS08160 at 1342.004 kb on - strand, within SGL_RS08160 at 1342.196 kb on + strand, within SGL_RS08165 at 1342.204 kb on - strand, within SGL_RS08165 at 1342.221 kb on - strand, within SGL_RS08165 at 1342.225 kb on + strand, within SGL_RS08165 at 1342.228 kb on - strand, within SGL_RS08165 at 1342.281 kb on - strand, within SGL_RS08165 at 1342.378 kb on - strand, within SGL_RS08165 at 1342.463 kb on + strand, within SGL_RS08165 at 1342.649 kb on + strand at 1342.649 kb on + strand at 1342.689 kb on + strand at 1342.818 kb on + strand at 1342.835 kb on + strand at 1342.849 kb on + strand at 1342.861 kb on + strand at 1342.867 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 150 uM remove 1,340,765 - SGL_RS08150 0.76 -0.5 1,340,815 + SGL_RS08150 0.79 -0.2 1,340,828 - SGL_RS08150 0.80 -0.0 1,340,870 - SGL_RS08150 0.83 +0.1 1,340,920 + SGL_RS08150 0.86 +0.3 1,341,246 - SGL_RS08155 0.23 -2.9 1,341,480 + SGL_RS08155 0.69 -3.4 1,341,524 + SGL_RS08155 0.78 -3.5 1,341,735 - SGL_RS08160 0.11 -1.6 1,341,852 + SGL_RS08160 0.43 -3.4 1,341,876 - SGL_RS08160 0.49 -1.2 1,341,882 - SGL_RS08160 0.51 -0.8 1,341,916 + SGL_RS08160 0.60 -3.2 1,341,998 - SGL_RS08160 0.82 -3.0 1,342,004 - SGL_RS08160 0.84 -3.8 1,342,196 + SGL_RS08165 0.24 +0.3 1,342,204 - SGL_RS08165 0.25 +0.3 1,342,221 - SGL_RS08165 0.28 -0.8 1,342,225 + SGL_RS08165 0.28 -0.4 1,342,228 - SGL_RS08165 0.29 -0.0 1,342,281 - SGL_RS08165 0.38 +0.4 1,342,378 - SGL_RS08165 0.54 +0.2 1,342,463 + SGL_RS08165 0.67 +1.1 1,342,649 + +0.8 1,342,649 + -0.3 1,342,689 + -0.2 1,342,818 + -0.1 1,342,835 + +0.1 1,342,849 + +0.1 1,342,861 + -0.1 1,342,867 - +0.0
Or see this region's nucleotide sequence