Experiment: Manganese (II) chloride tetrahydrate 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07540 and SGL_RS07545 are separated by 29 nucleotides SGL_RS07545 and SGL_RS07550 are separated by 54 nucleotides
SGL_RS07540: SGL_RS07540 - endonuclease MutS2, at 1,217,156 to 1,219,624
_RS07540
SGL_RS07545: SGL_RS07545 - serine/threonine-protein phosphatase, at 1,219,654 to 1,220,418
_RS07545
SGL_RS07550: SGL_RS07550 - AarF/ABC1/UbiB kinase family protein, at 1,220,473 to 1,222,230
_RS07550
Position (kb)
1219
1220
1221 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1218.676 kb on + strand, within SGL_RS07540 at 1218.937 kb on - strand, within SGL_RS07540 at 1219.021 kb on + strand, within SGL_RS07540 at 1219.211 kb on + strand, within SGL_RS07540 at 1219.298 kb on + strand, within SGL_RS07540 at 1219.632 kb on - strand at 1219.634 kb on - strand at 1219.660 kb on + strand at 1219.671 kb on + strand at 1219.765 kb on + strand, within SGL_RS07545 at 1219.833 kb on + strand, within SGL_RS07545 at 1219.833 kb on + strand, within SGL_RS07545 at 1219.841 kb on - strand, within SGL_RS07545 at 1219.928 kb on + strand, within SGL_RS07545 at 1219.934 kb on + strand, within SGL_RS07545 at 1219.942 kb on - strand, within SGL_RS07545 at 1219.957 kb on - strand, within SGL_RS07545 at 1220.109 kb on - strand, within SGL_RS07545 at 1220.260 kb on + strand, within SGL_RS07545 at 1220.322 kb on - strand, within SGL_RS07545 at 1220.465 kb on - strand at 1220.595 kb on - strand at 1220.659 kb on - strand, within SGL_RS07550 at 1220.690 kb on - strand, within SGL_RS07550 at 1220.695 kb on - strand, within SGL_RS07550 at 1220.732 kb on - strand, within SGL_RS07550 at 1220.763 kb on - strand, within SGL_RS07550 at 1220.828 kb on - strand, within SGL_RS07550 at 1221.002 kb on - strand, within SGL_RS07550 at 1221.089 kb on - strand, within SGL_RS07550 at 1221.108 kb on - strand, within SGL_RS07550 at 1221.198 kb on + strand, within SGL_RS07550 at 1221.206 kb on - strand, within SGL_RS07550 at 1221.301 kb on + strand, within SGL_RS07550
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 150 uM remove 1,218,676 + SGL_RS07540 0.62 -0.4 1,218,937 - SGL_RS07540 0.72 +0.3 1,219,021 + SGL_RS07540 0.76 -0.1 1,219,211 + SGL_RS07540 0.83 -0.2 1,219,298 + SGL_RS07540 0.87 -0.4 1,219,632 - -0.2 1,219,634 - -0.3 1,219,660 + -0.1 1,219,671 + -1.9 1,219,765 + SGL_RS07545 0.15 -2.0 1,219,833 + SGL_RS07545 0.23 -2.4 1,219,833 + SGL_RS07545 0.23 -1.7 1,219,841 - SGL_RS07545 0.24 -3.6 1,219,928 + SGL_RS07545 0.36 -2.5 1,219,934 + SGL_RS07545 0.37 -2.0 1,219,942 - SGL_RS07545 0.38 -2.5 1,219,957 - SGL_RS07545 0.40 -0.2 1,220,109 - SGL_RS07545 0.59 -2.9 1,220,260 + SGL_RS07545 0.79 -2.3 1,220,322 - SGL_RS07545 0.87 -1.0 1,220,465 - -1.6 1,220,595 - -0.2 1,220,659 - SGL_RS07550 0.11 -0.2 1,220,690 - SGL_RS07550 0.12 -0.2 1,220,695 - SGL_RS07550 0.13 -0.6 1,220,732 - SGL_RS07550 0.15 +0.1 1,220,763 - SGL_RS07550 0.16 -0.4 1,220,828 - SGL_RS07550 0.20 -0.2 1,221,002 - SGL_RS07550 0.30 -0.7 1,221,089 - SGL_RS07550 0.35 -1.2 1,221,108 - SGL_RS07550 0.36 +0.0 1,221,198 + SGL_RS07550 0.41 -0.3 1,221,206 - SGL_RS07550 0.42 -0.2 1,221,301 + SGL_RS07550 0.47 -0.2
Or see this region's nucleotide sequence