Experiment: Manganese (II) chloride tetrahydrate 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04400 and SGL_RS04405 overlap by 4 nucleotides SGL_RS04405 and SGL_RS04410 are separated by 59 nucleotides SGL_RS04410 and SGL_RS04415 are separated by 361 nucleotides
SGL_RS04400: SGL_RS04400 - carbon-nitrogen hydrolase family protein, at 545,591 to 546,556
_RS04400
SGL_RS04405: SGL_RS04405 - urease accessory protein UreD, at 546,553 to 547,365
_RS04405
SGL_RS04410: SGL_RS04410 - photosystem II protein PsbQ, at 547,425 to 547,874
_RS04410
SGL_RS04415: SGL_RS04415 - prohibitin family protein, at 548,236 to 549,132
_RS04415
Position (kb) 547
548 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 546.467 kb on - strand at 546.665 kb on - strand, within SGL_RS04405 at 546.776 kb on + strand, within SGL_RS04405 at 546.988 kb on - strand, within SGL_RS04405 at 547.044 kb on + strand, within SGL_RS04405 at 547.044 kb on + strand, within SGL_RS04405 at 547.074 kb on + strand, within SGL_RS04405 at 547.135 kb on + strand, within SGL_RS04405 at 547.147 kb on + strand, within SGL_RS04405 at 547.187 kb on + strand, within SGL_RS04405 at 547.192 kb on + strand, within SGL_RS04405 at 547.195 kb on - strand, within SGL_RS04405 at 547.212 kb on + strand, within SGL_RS04405 at 547.290 kb on + strand at 547.290 kb on + strand at 547.290 kb on - strand at 547.520 kb on - strand, within SGL_RS04410 at 547.578 kb on + strand, within SGL_RS04410 at 547.674 kb on + strand, within SGL_RS04410 at 547.773 kb on + strand, within SGL_RS04410 at 547.844 kb on - strand at 548.116 kb on - strand at 548.368 kb on + strand, within SGL_RS04415 at 548.462 kb on - strand, within SGL_RS04415 at 548.464 kb on + strand, within SGL_RS04415 at 548.466 kb on + strand, within SGL_RS04415 at 548.466 kb on + strand, within SGL_RS04415 at 548.466 kb on + strand, within SGL_RS04415 at 548.479 kb on - strand, within SGL_RS04415 at 548.616 kb on + strand, within SGL_RS04415 at 548.750 kb on + strand, within SGL_RS04415
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 150 uM remove 546,467 - +0.3 546,665 - SGL_RS04405 0.14 +0.2 546,776 + SGL_RS04405 0.27 -0.0 546,988 - SGL_RS04405 0.54 -0.1 547,044 + SGL_RS04405 0.60 +0.5 547,044 + SGL_RS04405 0.60 +0.2 547,074 + SGL_RS04405 0.64 -0.1 547,135 + SGL_RS04405 0.72 -0.1 547,147 + SGL_RS04405 0.73 +0.0 547,187 + SGL_RS04405 0.78 -0.7 547,192 + SGL_RS04405 0.79 +0.2 547,195 - SGL_RS04405 0.79 -0.9 547,212 + SGL_RS04405 0.81 -0.8 547,290 + -1.2 547,290 + -1.0 547,290 - -1.1 547,520 - SGL_RS04410 0.21 -3.0 547,578 + SGL_RS04410 0.34 -2.8 547,674 + SGL_RS04410 0.55 -2.5 547,773 + SGL_RS04410 0.77 -2.2 547,844 - -3.0 548,116 - +0.3 548,368 + SGL_RS04415 0.15 -0.4 548,462 - SGL_RS04415 0.25 -0.3 548,464 + SGL_RS04415 0.25 +0.1 548,466 + SGL_RS04415 0.26 +0.3 548,466 + SGL_RS04415 0.26 -0.0 548,466 + SGL_RS04415 0.26 +0.4 548,479 - SGL_RS04415 0.27 +0.7 548,616 + SGL_RS04415 0.42 -0.2 548,750 + SGL_RS04415 0.57 +0.1
Or see this region's nucleotide sequence