Experiment: Manganese (II) chloride tetrahydrate 150 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00235 and SGL_RS00240 are separated by 218 nucleotides SGL_RS00240 and SGL_RS00245 are separated by 44 nucleotides SGL_RS00245 and SGL_RS00250 are separated by 92 nucleotides SGL_RS00250 and SGL_RS00255 are separated by 0 nucleotides
SGL_RS00235: SGL_RS00235 - cellulose synthase operon protein YhjQ/BcsQ, at 50,111 to 52,333
_RS00235
SGL_RS00240: SGL_RS00240 - cyanoexosortase A system-associated protein, at 52,552 to 52,992
_RS00240
SGL_RS00245: SGL_RS00245 - glycosyltransferase, at 53,037 to 54,026
_RS00245
SGL_RS00250: SGL_RS00250 - WecB/TagA/CpsF family glycosyltransferase, at 54,119 to 54,910
_RS00250
SGL_RS00255: SGL_RS00255 - glycosyltransferase family 2 protein, at 54,911 to 56,281
_RS00255
Position (kb)
53
54
55 Strain fitness (log2 ratio)
-1
0
1 at 52.138 kb on - strand at 52.222 kb on + strand at 52.276 kb on - strand at 52.365 kb on - strand at 52.380 kb on + strand at 52.382 kb on + strand at 52.429 kb on - strand at 52.513 kb on - strand at 52.515 kb on + strand at 52.515 kb on + strand at 52.515 kb on + strand at 52.515 kb on + strand at 52.545 kb on - strand at 52.593 kb on + strand at 52.620 kb on + strand, within SGL_RS00240 at 52.620 kb on + strand, within SGL_RS00240 at 52.706 kb on + strand, within SGL_RS00240 at 52.868 kb on - strand, within SGL_RS00240 at 52.927 kb on + strand, within SGL_RS00240 at 52.953 kb on + strand at 52.958 kb on + strand at 52.958 kb on + strand at 52.958 kb on + strand at 52.961 kb on - strand at 52.966 kb on - strand at 52.966 kb on - strand at 52.966 kb on - strand at 52.986 kb on + strand at 52.994 kb on - strand at 52.994 kb on - strand at 53.002 kb on + strand at 53.025 kb on + strand at 53.083 kb on + strand at 53.113 kb on + strand at 53.190 kb on - strand, within SGL_RS00245 at 53.273 kb on - strand, within SGL_RS00245 at 53.283 kb on - strand, within SGL_RS00245 at 53.357 kb on + strand, within SGL_RS00245 at 53.395 kb on - strand, within SGL_RS00245 at 53.396 kb on + strand, within SGL_RS00245 at 53.426 kb on + strand, within SGL_RS00245 at 53.426 kb on + strand, within SGL_RS00245 at 53.452 kb on - strand, within SGL_RS00245 at 53.452 kb on - strand, within SGL_RS00245 at 53.514 kb on + strand, within SGL_RS00245 at 53.540 kb on - strand, within SGL_RS00245 at 53.559 kb on - strand, within SGL_RS00245 at 53.561 kb on + strand, within SGL_RS00245 at 53.561 kb on - strand, within SGL_RS00245 at 53.669 kb on - strand, within SGL_RS00245 at 53.731 kb on + strand, within SGL_RS00245 at 53.749 kb on + strand, within SGL_RS00245 at 53.828 kb on + strand, within SGL_RS00245 at 53.971 kb on + strand at 54.068 kb on - strand at 54.209 kb on - strand, within SGL_RS00250 at 54.608 kb on - strand, within SGL_RS00250 at 54.608 kb on - strand, within SGL_RS00250 at 54.638 kb on - strand, within SGL_RS00250 at 54.686 kb on + strand, within SGL_RS00250 at 54.859 kb on + strand at 54.859 kb on + strand at 54.868 kb on + strand at 54.888 kb on - strand at 54.905 kb on + strand at 54.929 kb on + strand at 54.956 kb on + strand at 54.966 kb on + strand at 54.966 kb on + strand at 54.966 kb on + strand at 54.974 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Manganese (II) chloride tetrahydrate 150 uM remove 52,138 - +0.1 52,222 + -0.2 52,276 - -0.2 52,365 - -0.3 52,380 + +0.0 52,382 + +0.6 52,429 - -0.3 52,513 - +0.1 52,515 + -0.1 52,515 + +0.2 52,515 + -0.1 52,515 + +0.1 52,545 - -0.9 52,593 + +0.2 52,620 + SGL_RS00240 0.15 -0.4 52,620 + SGL_RS00240 0.15 +0.3 52,706 + SGL_RS00240 0.35 -0.7 52,868 - SGL_RS00240 0.72 +0.2 52,927 + SGL_RS00240 0.85 -0.0 52,953 + +0.6 52,958 + -0.2 52,958 + +0.9 52,958 + -0.2 52,961 - +0.0 52,966 - +0.0 52,966 - -0.1 52,966 - -0.3 52,986 + +0.4 52,994 - +0.0 52,994 - -0.1 53,002 + -0.4 53,025 + -0.2 53,083 + -0.6 53,113 + -1.0 53,190 - SGL_RS00245 0.15 -1.2 53,273 - SGL_RS00245 0.24 -0.0 53,283 - SGL_RS00245 0.25 -0.8 53,357 + SGL_RS00245 0.32 -0.8 53,395 - SGL_RS00245 0.36 -0.9 53,396 + SGL_RS00245 0.36 -0.8 53,426 + SGL_RS00245 0.39 -0.8 53,426 + SGL_RS00245 0.39 -0.1 53,452 - SGL_RS00245 0.42 -0.4 53,452 - SGL_RS00245 0.42 -1.2 53,514 + SGL_RS00245 0.48 -0.5 53,540 - SGL_RS00245 0.51 -1.5 53,559 - SGL_RS00245 0.53 -1.2 53,561 + SGL_RS00245 0.53 -1.2 53,561 - SGL_RS00245 0.53 -0.7 53,669 - SGL_RS00245 0.64 -1.0 53,731 + SGL_RS00245 0.70 -1.1 53,749 + SGL_RS00245 0.72 -1.1 53,828 + SGL_RS00245 0.80 -1.1 53,971 + +0.1 54,068 - -0.0 54,209 - SGL_RS00250 0.11 -0.0 54,608 - SGL_RS00250 0.62 -0.7 54,608 - SGL_RS00250 0.62 -0.4 54,638 - SGL_RS00250 0.66 -0.3 54,686 + SGL_RS00250 0.72 -0.2 54,859 + +0.2 54,859 + +0.3 54,868 + -0.1 54,888 - +0.1 54,905 + -0.4 54,929 + +0.1 54,956 + +0.0 54,966 + -0.3 54,966 + +0.4 54,966 + +0.4 54,974 - -0.1
Or see this region's nucleotide sequence