Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS17300 and SGL_RS17305 are separated by 133 nucleotides SGL_RS17305 and SGL_RS17310 are separated by 14 nucleotides
SGL_RS17300: SGL_RS17300 - M3 family metallopeptidase, at 3,336,464 to 3,338,548
_RS17300
SGL_RS17305: SGL_RS17305 - pyrroline-5-carboxylate reductase, at 3,338,682 to 3,339,485
_RS17305
SGL_RS17310: SGL_RS17310 - glycoside hydrolase 100 family protein, at 3,339,500 to 3,340,969
_RS17310
Position (kb)
3338
3339
3340 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3338.027 kb on - strand, within SGL_RS17300 at 3338.071 kb on - strand, within SGL_RS17300 at 3338.162 kb on + strand, within SGL_RS17300 at 3338.294 kb on + strand, within SGL_RS17300 at 3338.354 kb on - strand at 3338.378 kb on + strand at 3338.536 kb on - strand at 3338.538 kb on + strand at 3338.546 kb on - strand at 3338.668 kb on - strand at 3338.668 kb on - strand at 3338.859 kb on - strand, within SGL_RS17305 at 3338.912 kb on - strand, within SGL_RS17305 at 3339.007 kb on - strand, within SGL_RS17305 at 3339.032 kb on + strand, within SGL_RS17305 at 3339.113 kb on + strand, within SGL_RS17305 at 3339.113 kb on + strand, within SGL_RS17305 at 3339.137 kb on + strand, within SGL_RS17305 at 3339.255 kb on + strand, within SGL_RS17305 at 3339.367 kb on + strand, within SGL_RS17305 at 3339.553 kb on - strand at 3339.819 kb on - strand, within SGL_RS17310 at 3339.922 kb on - strand, within SGL_RS17310 at 3339.950 kb on + strand, within SGL_RS17310 at 3340.169 kb on - strand, within SGL_RS17310 at 3340.280 kb on + strand, within SGL_RS17310 at 3340.313 kb on + strand, within SGL_RS17310 at 3340.448 kb on + strand, within SGL_RS17310
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 3,338,027 - SGL_RS17300 0.75 +0.8 3,338,071 - SGL_RS17300 0.77 -1.3 3,338,162 + SGL_RS17300 0.81 -0.9 3,338,294 + SGL_RS17300 0.88 +0.3 3,338,354 - +0.1 3,338,378 + -0.2 3,338,536 - -0.2 3,338,538 + -0.2 3,338,546 - +0.2 3,338,668 - -0.3 3,338,668 - -3.6 3,338,859 - SGL_RS17305 0.22 -2.0 3,338,912 - SGL_RS17305 0.29 -3.0 3,339,007 - SGL_RS17305 0.40 -4.4 3,339,032 + SGL_RS17305 0.44 -2.2 3,339,113 + SGL_RS17305 0.54 -2.3 3,339,113 + SGL_RS17305 0.54 -3.3 3,339,137 + SGL_RS17305 0.57 -3.9 3,339,255 + SGL_RS17305 0.71 -4.1 3,339,367 + SGL_RS17305 0.85 -3.2 3,339,553 - -0.2 3,339,819 - SGL_RS17310 0.22 -0.1 3,339,922 - SGL_RS17310 0.29 +0.6 3,339,950 + SGL_RS17310 0.31 +0.6 3,340,169 - SGL_RS17310 0.46 +0.3 3,340,280 + SGL_RS17310 0.53 +1.0 3,340,313 + SGL_RS17310 0.55 -0.2 3,340,448 + SGL_RS17310 0.64 +0.2
Or see this region's nucleotide sequence