Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16695 and SGL_RS16700 are separated by 131 nucleotides SGL_RS16700 and SGL_RS16705 overlap by 4 nucleotides SGL_RS16705 and SGL_RS16710 are separated by 42 nucleotides
SGL_RS16695: SGL_RS16695 - magnesium/cobalt transporter CorA, at 3,202,699 to 3,203,862
_RS16695
SGL_RS16700: SGL_RS16700 - polyprenyl diphosphate synthase, at 3,203,994 to 3,204,743
_RS16700
SGL_RS16705: SGL_RS16705 - diadenylate cyclase CdaA, at 3,204,740 to 3,205,651
_RS16705
SGL_RS16710: SGL_RS16710 - diaminopimelate decarboxylase, at 3,205,694 to 3,207,103
_RS16710
Position (kb) 3204
3205
3206 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 3203.770 kb on - strand at 3203.786 kb on + strand at 3203.928 kb on - strand at 3203.992 kb on + strand at 3204.954 kb on - strand, within SGL_RS16705 at 3205.329 kb on - strand, within SGL_RS16705 at 3205.329 kb on - strand, within SGL_RS16705 at 3205.364 kb on + strand, within SGL_RS16705 at 3205.415 kb on + strand, within SGL_RS16705 at 3205.423 kb on - strand, within SGL_RS16705 at 3205.430 kb on - strand, within SGL_RS16705 at 3205.707 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 3,203,770 - +0.3 3,203,786 + +0.7 3,203,928 - -0.3 3,203,992 + -0.5 3,204,954 - SGL_RS16705 0.23 -2.3 3,205,329 - SGL_RS16705 0.65 -2.2 3,205,329 - SGL_RS16705 0.65 -1.3 3,205,364 + SGL_RS16705 0.68 -4.9 3,205,415 + SGL_RS16705 0.74 -4.1 3,205,423 - SGL_RS16705 0.75 -2.0 3,205,430 - SGL_RS16705 0.76 -2.9 3,205,707 - -0.1
Or see this region's nucleotide sequence