Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16540 and SGL_RS16545 are separated by 160 nucleotides SGL_RS16545 and SGL_RS16550 are separated by 458 nucleotides
SGL_RS16540: SGL_RS16540 - carotene isomerase, at 3,162,738 to 3,164,243
_RS16540
SGL_RS16545: SGL_RS16545 - LdpA C-terminal domain-containing domain, at 3,164,404 to 3,165,591
_RS16545
SGL_RS16550: SGL_RS16550 - hypothetical protein, at 3,166,050 to 3,166,448
_RS16550
Position (kb)
3164
3165
3166 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3163.554 kb on - strand, within SGL_RS16540 at 3163.622 kb on - strand, within SGL_RS16540 at 3163.685 kb on + strand, within SGL_RS16540 at 3163.692 kb on + strand, within SGL_RS16540 at 3163.745 kb on - strand, within SGL_RS16540 at 3163.790 kb on + strand, within SGL_RS16540 at 3163.790 kb on + strand, within SGL_RS16540 at 3163.790 kb on + strand, within SGL_RS16540 at 3163.798 kb on - strand, within SGL_RS16540 at 3163.824 kb on + strand, within SGL_RS16540 at 3163.832 kb on - strand, within SGL_RS16540 at 3163.871 kb on - strand, within SGL_RS16540 at 3164.035 kb on - strand, within SGL_RS16540 at 3164.172 kb on - strand at 3164.182 kb on - strand at 3164.208 kb on + strand at 3164.239 kb on - strand at 3164.271 kb on + strand at 3164.287 kb on + strand at 3164.382 kb on - strand at 3164.533 kb on - strand, within SGL_RS16545 at 3164.539 kb on + strand, within SGL_RS16545 at 3164.608 kb on + strand, within SGL_RS16545 at 3164.679 kb on - strand, within SGL_RS16545 at 3164.737 kb on + strand, within SGL_RS16545 at 3164.742 kb on + strand, within SGL_RS16545 at 3164.747 kb on + strand, within SGL_RS16545 at 3164.763 kb on + strand, within SGL_RS16545 at 3164.764 kb on - strand, within SGL_RS16545 at 3164.792 kb on + strand, within SGL_RS16545 at 3164.792 kb on + strand, within SGL_RS16545 at 3164.838 kb on + strand, within SGL_RS16545 at 3164.949 kb on - strand, within SGL_RS16545 at 3164.949 kb on - strand, within SGL_RS16545 at 3165.084 kb on - strand, within SGL_RS16545 at 3165.094 kb on - strand, within SGL_RS16545 at 3165.133 kb on + strand, within SGL_RS16545 at 3165.152 kb on + strand, within SGL_RS16545 at 3165.188 kb on - strand, within SGL_RS16545 at 3165.200 kb on - strand, within SGL_RS16545 at 3165.369 kb on - strand, within SGL_RS16545 at 3165.654 kb on - strand at 3165.654 kb on - strand at 3165.658 kb on + strand at 3165.672 kb on - strand at 3165.763 kb on + strand at 3165.912 kb on + strand at 3165.912 kb on + strand at 3166.016 kb on + strand at 3166.156 kb on + strand, within SGL_RS16550 at 3166.441 kb on + strand at 3166.460 kb on + strand at 3166.502 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 3,163,554 - SGL_RS16540 0.54 -0.8 3,163,622 - SGL_RS16540 0.59 -1.3 3,163,685 + SGL_RS16540 0.63 -0.4 3,163,692 + SGL_RS16540 0.63 -0.6 3,163,745 - SGL_RS16540 0.67 -0.8 3,163,790 + SGL_RS16540 0.70 -0.7 3,163,790 + SGL_RS16540 0.70 +0.0 3,163,790 + SGL_RS16540 0.70 -0.7 3,163,798 - SGL_RS16540 0.70 -0.2 3,163,824 + SGL_RS16540 0.72 -0.2 3,163,832 - SGL_RS16540 0.73 -0.5 3,163,871 - SGL_RS16540 0.75 -0.7 3,164,035 - SGL_RS16540 0.86 -0.0 3,164,172 - -0.6 3,164,182 - -0.9 3,164,208 + -0.9 3,164,239 - -0.3 3,164,271 + -0.3 3,164,287 + -0.8 3,164,382 - -4.1 3,164,533 - SGL_RS16545 0.11 -1.8 3,164,539 + SGL_RS16545 0.11 -1.5 3,164,608 + SGL_RS16545 0.17 -0.8 3,164,679 - SGL_RS16545 0.23 -1.4 3,164,737 + SGL_RS16545 0.28 -2.3 3,164,742 + SGL_RS16545 0.28 -0.5 3,164,747 + SGL_RS16545 0.29 -1.9 3,164,763 + SGL_RS16545 0.30 -1.7 3,164,764 - SGL_RS16545 0.30 -0.8 3,164,792 + SGL_RS16545 0.33 -1.4 3,164,792 + SGL_RS16545 0.33 -0.5 3,164,838 + SGL_RS16545 0.37 -0.9 3,164,949 - SGL_RS16545 0.46 -1.1 3,164,949 - SGL_RS16545 0.46 -0.9 3,165,084 - SGL_RS16545 0.57 -1.2 3,165,094 - SGL_RS16545 0.58 -1.6 3,165,133 + SGL_RS16545 0.61 -1.8 3,165,152 + SGL_RS16545 0.63 -1.4 3,165,188 - SGL_RS16545 0.66 -1.6 3,165,200 - SGL_RS16545 0.67 -0.7 3,165,369 - SGL_RS16545 0.81 -1.4 3,165,654 - -0.4 3,165,654 - -0.0 3,165,658 + -0.0 3,165,672 - -0.0 3,165,763 + +0.2 3,165,912 + -1.0 3,165,912 + -3.2 3,166,016 + -1.3 3,166,156 + SGL_RS16550 0.27 -2.7 3,166,441 + -0.3 3,166,460 + +0.3 3,166,502 + -3.3
Or see this region's nucleotide sequence