Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS15860 and SGL_RS15865 are separated by 57 nucleotides SGL_RS15865 and SGL_RS15870 are separated by 76 nucleotides SGL_RS15870 and SGL_RS15875 are separated by 211 nucleotides SGL_RS15875 and SGL_RS15880 are separated by 61 nucleotides
SGL_RS15860: SGL_RS15860 - cell division topological specificity factor MinE, at 3,007,280 to 3,007,573
_RS15860
SGL_RS15865: SGL_RS15865 - septum site-determining protein MinD, at 3,007,631 to 3,008,431
_RS15865
SGL_RS15870: SGL_RS15870 - septum site-determining protein MinC, at 3,008,508 to 3,009,251
_RS15870
SGL_RS15875: SGL_RS15875 - FHA domain-containing protein, at 3,009,463 to 3,010,095
_RS15875
SGL_RS15880: SGL_RS15880 - CatA-like O-acetyltransferase, at 3,010,157 to 3,010,864
_RS15880
Position (kb)
3008
3009
3010 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3007.585 kb on - strand at 3007.599 kb on - strand at 3007.635 kb on + strand at 3007.679 kb on - strand at 3007.679 kb on - strand at 3007.783 kb on - strand, within SGL_RS15865 at 3007.985 kb on - strand, within SGL_RS15865 at 3007.996 kb on + strand, within SGL_RS15865 at 3008.348 kb on + strand, within SGL_RS15865 at 3008.416 kb on + strand at 3008.472 kb on - strand at 3008.627 kb on - strand, within SGL_RS15870 at 3008.708 kb on - strand, within SGL_RS15870 at 3008.763 kb on - strand, within SGL_RS15870 at 3008.784 kb on + strand, within SGL_RS15870 at 3008.836 kb on - strand, within SGL_RS15870 at 3008.836 kb on - strand, within SGL_RS15870 at 3008.866 kb on - strand, within SGL_RS15870 at 3008.896 kb on + strand, within SGL_RS15870 at 3008.909 kb on - strand, within SGL_RS15870 at 3008.958 kb on + strand, within SGL_RS15870 at 3008.970 kb on + strand, within SGL_RS15870 at 3009.143 kb on + strand, within SGL_RS15870 at 3009.143 kb on + strand, within SGL_RS15870 at 3009.151 kb on - strand, within SGL_RS15870 at 3009.151 kb on - strand, within SGL_RS15870 at 3009.229 kb on + strand at 3009.229 kb on + strand at 3009.237 kb on - strand at 3009.291 kb on - strand at 3009.291 kb on - strand at 3009.291 kb on - strand at 3009.320 kb on + strand at 3009.328 kb on - strand at 3009.384 kb on - strand at 3009.532 kb on - strand, within SGL_RS15875 at 3009.535 kb on - strand, within SGL_RS15875 at 3009.542 kb on + strand, within SGL_RS15875 at 3009.550 kb on - strand, within SGL_RS15875 at 3009.557 kb on - strand, within SGL_RS15875 at 3009.564 kb on + strand, within SGL_RS15875 at 3009.574 kb on + strand, within SGL_RS15875 at 3009.655 kb on + strand, within SGL_RS15875 at 3009.707 kb on - strand, within SGL_RS15875 at 3009.832 kb on - strand, within SGL_RS15875 at 3009.838 kb on - strand, within SGL_RS15875 at 3009.909 kb on + strand, within SGL_RS15875 at 3009.979 kb on - strand, within SGL_RS15875 at 3010.000 kb on + strand, within SGL_RS15875 at 3010.008 kb on - strand, within SGL_RS15875 at 3010.029 kb on - strand, within SGL_RS15875 at 3010.036 kb on + strand at 3010.081 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 3,007,585 - -1.3 3,007,599 - -1.0 3,007,635 + -3.2 3,007,679 - -1.6 3,007,679 - -0.7 3,007,783 - SGL_RS15865 0.19 -2.3 3,007,985 - SGL_RS15865 0.44 -3.1 3,007,996 + SGL_RS15865 0.46 -3.3 3,008,348 + SGL_RS15865 0.90 -1.4 3,008,416 + -2.3 3,008,472 - -1.8 3,008,627 - SGL_RS15870 0.16 -3.2 3,008,708 - SGL_RS15870 0.27 -1.2 3,008,763 - SGL_RS15870 0.34 -2.0 3,008,784 + SGL_RS15870 0.37 -2.3 3,008,836 - SGL_RS15870 0.44 -3.3 3,008,836 - SGL_RS15870 0.44 -0.6 3,008,866 - SGL_RS15870 0.48 -2.7 3,008,896 + SGL_RS15870 0.52 -1.3 3,008,909 - SGL_RS15870 0.54 -1.6 3,008,958 + SGL_RS15870 0.60 -1.8 3,008,970 + SGL_RS15870 0.62 -1.2 3,009,143 + SGL_RS15870 0.85 -3.0 3,009,143 + SGL_RS15870 0.85 -1.8 3,009,151 - SGL_RS15870 0.86 -1.2 3,009,151 - SGL_RS15870 0.86 -1.2 3,009,229 + -2.0 3,009,229 + -2.1 3,009,237 - +1.0 3,009,291 - +0.4 3,009,291 - -1.5 3,009,291 - -0.5 3,009,320 + +0.1 3,009,328 - -0.2 3,009,384 - -0.1 3,009,532 - SGL_RS15875 0.11 -1.7 3,009,535 - SGL_RS15875 0.11 -0.7 3,009,542 + SGL_RS15875 0.12 -1.5 3,009,550 - SGL_RS15875 0.14 -1.6 3,009,557 - SGL_RS15875 0.15 -1.2 3,009,564 + SGL_RS15875 0.16 -1.4 3,009,574 + SGL_RS15875 0.18 -1.7 3,009,655 + SGL_RS15875 0.30 -1.4 3,009,707 - SGL_RS15875 0.39 -1.5 3,009,832 - SGL_RS15875 0.58 -2.6 3,009,838 - SGL_RS15875 0.59 -3.4 3,009,909 + SGL_RS15875 0.70 +0.8 3,009,979 - SGL_RS15875 0.82 -2.5 3,010,000 + SGL_RS15875 0.85 -0.5 3,010,008 - SGL_RS15875 0.86 -2.3 3,010,029 - SGL_RS15875 0.89 -3.4 3,010,036 + -1.6 3,010,081 - -2.2
Or see this region's nucleotide sequence