Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14575 and SGL_RS14580 are separated by 222 nucleotides SGL_RS14580 and SGL_RS14585 are separated by 55 nucleotides SGL_RS14585 and SGL_RS14590 are separated by 56 nucleotides SGL_RS14590 and SGL_RS14595 overlap by 13 nucleotides
SGL_RS14575: SGL_RS14575 - RNA-binding protein, at 2,724,648 to 2,725,103
_RS14575
SGL_RS14580: SGL_RS14580 - ribose-5-phosphate isomerase RpiA, at 2,725,326 to 2,726,033
_RS14580
SGL_RS14585: SGL_RS14585 - TolB family protein, at 2,726,089 to 2,726,640
_RS14585
SGL_RS14590: SGL_RS14590 - hypothetical protein, at 2,726,697 to 2,727,059
_RS14590
SGL_RS14595: SGL_RS14595 - phospholipase D-like domain-containing protein, at 2,727,047 to 2,728,708
_RS14595
Position (kb)
2726
2727 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2725.129 kb on - strand at 2725.529 kb on - strand, within SGL_RS14580 at 2725.652 kb on - strand, within SGL_RS14580 at 2726.060 kb on - strand at 2726.241 kb on - strand, within SGL_RS14585 at 2726.383 kb on - strand, within SGL_RS14585 at 2726.837 kb on - strand, within SGL_RS14590 at 2726.959 kb on + strand, within SGL_RS14590 at 2727.098 kb on + strand at 2727.106 kb on - strand at 2727.151 kb on + strand at 2727.156 kb on + strand at 2727.175 kb on - strand at 2727.184 kb on + strand at 2727.192 kb on - strand at 2727.374 kb on + strand, within SGL_RS14595 at 2727.457 kb on - strand, within SGL_RS14595 at 2727.484 kb on + strand, within SGL_RS14595 at 2727.497 kb on + strand, within SGL_RS14595 at 2727.549 kb on + strand, within SGL_RS14595 at 2727.588 kb on + strand, within SGL_RS14595 at 2727.640 kb on - strand, within SGL_RS14595
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 2,725,129 - -0.2 2,725,529 - SGL_RS14580 0.29 -2.3 2,725,652 - SGL_RS14580 0.46 -3.7 2,726,060 - +0.2 2,726,241 - SGL_RS14585 0.28 -1.6 2,726,383 - SGL_RS14585 0.53 -2.2 2,726,837 - SGL_RS14590 0.39 +0.2 2,726,959 + SGL_RS14590 0.72 +0.1 2,727,098 + -0.2 2,727,106 - +0.1 2,727,151 + -0.0 2,727,156 + -0.2 2,727,175 - +0.3 2,727,184 + +0.0 2,727,192 - +0.1 2,727,374 + SGL_RS14595 0.20 -0.1 2,727,457 - SGL_RS14595 0.25 -0.1 2,727,484 + SGL_RS14595 0.26 +0.5 2,727,497 + SGL_RS14595 0.27 -0.1 2,727,549 + SGL_RS14595 0.30 -0.5 2,727,588 + SGL_RS14595 0.33 -0.2 2,727,640 - SGL_RS14595 0.36 +0.0
Or see this region's nucleotide sequence