Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14295 and SGL_RS14300 are separated by 214 nucleotides SGL_RS14300 and SGL_RS14305 are separated by 81 nucleotides SGL_RS14305 and SGL_RS14310 overlap by 46 nucleotides
SGL_RS14295: SGL_RS14295 - mechanosensitive ion channel, at 2,660,854 to 2,661,744
_RS14295
SGL_RS14300: SGL_RS14300 - HAMP domain-containing sensor histidine kinase, at 2,661,959 to 2,663,284
_RS14300
SGL_RS14305: SGL_RS14305 - DUF3593 domain-containing protein, at 2,663,366 to 2,663,731
_RS14305
SGL_RS14310: SGL_RS14310 - DUF2499 domain-containing protein, at 2,663,686 to 2,664,000
_RS14310
Position (kb)
2661
2662
2663
2664 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2661.969 kb on - strand at 2661.981 kb on - strand at 2662.094 kb on - strand, within SGL_RS14300 at 2662.106 kb on - strand, within SGL_RS14300 at 2662.141 kb on - strand, within SGL_RS14300 at 2662.181 kb on - strand, within SGL_RS14300 at 2662.302 kb on - strand, within SGL_RS14300 at 2662.373 kb on + strand, within SGL_RS14300 at 2662.373 kb on + strand, within SGL_RS14300 at 2662.381 kb on - strand, within SGL_RS14300 at 2662.381 kb on - strand, within SGL_RS14300 at 2662.381 kb on - strand, within SGL_RS14300 at 2662.627 kb on - strand, within SGL_RS14300 at 2662.644 kb on + strand, within SGL_RS14300 at 2662.652 kb on - strand, within SGL_RS14300 at 2662.657 kb on - strand, within SGL_RS14300 at 2662.680 kb on + strand, within SGL_RS14300 at 2662.734 kb on - strand, within SGL_RS14300 at 2662.947 kb on + strand, within SGL_RS14300 at 2662.954 kb on - strand, within SGL_RS14300 at 2662.974 kb on + strand, within SGL_RS14300 at 2662.994 kb on - strand, within SGL_RS14300 at 2662.998 kb on + strand, within SGL_RS14300 at 2663.005 kb on + strand, within SGL_RS14300 at 2663.008 kb on + strand, within SGL_RS14300 at 2663.028 kb on - strand, within SGL_RS14300 at 2663.081 kb on + strand, within SGL_RS14300 at 2663.091 kb on - strand, within SGL_RS14300 at 2663.195 kb on - strand at 2663.239 kb on - strand at 2663.320 kb on + strand at 2663.473 kb on - strand, within SGL_RS14305 at 2663.522 kb on - strand, within SGL_RS14305 at 2663.770 kb on + strand, within SGL_RS14310 at 2663.770 kb on + strand, within SGL_RS14310 at 2663.784 kb on - strand, within SGL_RS14310 at 2663.787 kb on + strand, within SGL_RS14310 at 2663.844 kb on - strand, within SGL_RS14310 at 2663.870 kb on + strand, within SGL_RS14310 at 2663.875 kb on + strand, within SGL_RS14310 at 2663.875 kb on + strand, within SGL_RS14310 at 2663.875 kb on + strand, within SGL_RS14310 at 2663.875 kb on + strand, within SGL_RS14310 at 2663.878 kb on - strand, within SGL_RS14310 at 2663.878 kb on - strand, within SGL_RS14310 at 2663.893 kb on + strand, within SGL_RS14310 at 2663.901 kb on - strand, within SGL_RS14310 at 2663.901 kb on - strand, within SGL_RS14310 at 2663.909 kb on + strand, within SGL_RS14310 at 2663.921 kb on - strand, within SGL_RS14310 at 2664.068 kb on + strand at 2664.076 kb on - strand at 2664.111 kb on + strand at 2664.249 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 2,661,969 - -1.2 2,661,981 - -1.9 2,662,094 - SGL_RS14300 0.10 -2.0 2,662,106 - SGL_RS14300 0.11 -2.3 2,662,141 - SGL_RS14300 0.14 -2.7 2,662,181 - SGL_RS14300 0.17 -2.5 2,662,302 - SGL_RS14300 0.26 -2.0 2,662,373 + SGL_RS14300 0.31 -2.1 2,662,373 + SGL_RS14300 0.31 -2.2 2,662,381 - SGL_RS14300 0.32 -2.3 2,662,381 - SGL_RS14300 0.32 -1.0 2,662,381 - SGL_RS14300 0.32 -5.1 2,662,627 - SGL_RS14300 0.50 -2.5 2,662,644 + SGL_RS14300 0.52 -1.8 2,662,652 - SGL_RS14300 0.52 -2.0 2,662,657 - SGL_RS14300 0.53 -2.2 2,662,680 + SGL_RS14300 0.54 -3.7 2,662,734 - SGL_RS14300 0.58 -1.7 2,662,947 + SGL_RS14300 0.75 -1.3 2,662,954 - SGL_RS14300 0.75 -1.2 2,662,974 + SGL_RS14300 0.77 -3.8 2,662,994 - SGL_RS14300 0.78 -2.0 2,662,998 + SGL_RS14300 0.78 -1.7 2,663,005 + SGL_RS14300 0.79 -2.9 2,663,008 + SGL_RS14300 0.79 -2.6 2,663,028 - SGL_RS14300 0.81 -2.4 2,663,081 + SGL_RS14300 0.85 -3.0 2,663,091 - SGL_RS14300 0.85 -1.7 2,663,195 - -2.6 2,663,239 - -1.9 2,663,320 + -0.3 2,663,473 - SGL_RS14305 0.29 +0.3 2,663,522 - SGL_RS14305 0.43 -0.2 2,663,770 + SGL_RS14310 0.27 -0.1 2,663,770 + SGL_RS14310 0.27 +0.2 2,663,784 - SGL_RS14310 0.31 -0.0 2,663,787 + SGL_RS14310 0.32 +0.2 2,663,844 - SGL_RS14310 0.50 -0.1 2,663,870 + SGL_RS14310 0.58 +0.1 2,663,875 + SGL_RS14310 0.60 -0.0 2,663,875 + SGL_RS14310 0.60 +0.1 2,663,875 + SGL_RS14310 0.60 -0.2 2,663,875 + SGL_RS14310 0.60 +0.2 2,663,878 - SGL_RS14310 0.61 -0.4 2,663,878 - SGL_RS14310 0.61 +0.2 2,663,893 + SGL_RS14310 0.66 +0.3 2,663,901 - SGL_RS14310 0.68 -0.1 2,663,901 - SGL_RS14310 0.68 -0.0 2,663,909 + SGL_RS14310 0.71 +0.2 2,663,921 - SGL_RS14310 0.75 +0.2 2,664,068 + +0.5 2,664,076 - -0.3 2,664,111 + -0.1 2,664,249 - -0.1
Or see this region's nucleotide sequence