Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12860 and SGL_RS12865 are separated by 37 nucleotides SGL_RS12865 and SGL_RS12870 are separated by 26 nucleotides SGL_RS12870 and SGL_RS12875 are separated by 372 nucleotides
SGL_RS12860: SGL_RS12860 - NCS2 family permease, at 2,344,152 to 2,345,759
_RS12860
SGL_RS12865: SGL_RS12865 - pseudouridine synthase, at 2,345,797 to 2,346,546
_RS12865
SGL_RS12870: SGL_RS12870 - YqeG family HAD IIIA-type phosphatase, at 2,346,573 to 2,347,127
_RS12870
SGL_RS12875: SGL_RS12875 - DUF3769 domain-containing protein, at 2,347,500 to 2,349,911
_RS12875
Position (kb)
2346
2347
2348 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2345.575 kb on + strand, within SGL_RS12860 at 2345.666 kb on - strand at 2345.671 kb on - strand at 2345.748 kb on + strand at 2345.795 kb on - strand at 2346.100 kb on - strand, within SGL_RS12865 at 2346.217 kb on - strand, within SGL_RS12865 at 2346.236 kb on - strand, within SGL_RS12865 at 2346.297 kb on + strand, within SGL_RS12865 at 2346.434 kb on + strand, within SGL_RS12865 at 2346.531 kb on + strand at 2346.670 kb on - strand, within SGL_RS12870 at 2346.689 kb on + strand, within SGL_RS12870 at 2346.690 kb on + strand, within SGL_RS12870 at 2346.691 kb on - strand, within SGL_RS12870 at 2346.888 kb on - strand, within SGL_RS12870 at 2347.093 kb on + strand at 2347.101 kb on - strand at 2347.104 kb on + strand at 2347.488 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 2,345,575 + SGL_RS12860 0.88 -0.0 2,345,666 - +0.2 2,345,671 - +0.1 2,345,748 + -0.4 2,345,795 - -0.1 2,346,100 - SGL_RS12865 0.40 +0.2 2,346,217 - SGL_RS12865 0.56 +0.5 2,346,236 - SGL_RS12865 0.59 +0.1 2,346,297 + SGL_RS12865 0.67 -0.1 2,346,434 + SGL_RS12865 0.85 +0.1 2,346,531 + +1.8 2,346,670 - SGL_RS12870 0.17 -5.5 2,346,689 + SGL_RS12870 0.21 -5.4 2,346,690 + SGL_RS12870 0.21 -4.0 2,346,691 - SGL_RS12870 0.21 -3.4 2,346,888 - SGL_RS12870 0.57 -5.1 2,347,093 + -0.3 2,347,101 - -1.0 2,347,104 + -1.2 2,347,488 - +0.0
Or see this region's nucleotide sequence