Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12750 and SGL_RS12755 are separated by 205 nucleotides SGL_RS12755 and SGL_RS12760 are separated by 187 nucleotides SGL_RS12760 and SGL_RS12765 are separated by 88 nucleotides
SGL_RS12750: SGL_RS12750 - molecular chaperone DnaK, at 2,312,664 to 2,314,574
_RS12750
SGL_RS12755: SGL_RS12755 - IMS domain-containing protein, at 2,314,780 to 2,316,924
_RS12755
SGL_RS12760: SGL_RS12760 - hypothetical protein, at 2,317,112 to 2,317,447
_RS12760
SGL_RS12765: SGL_RS12765 - hypothetical protein, at 2,317,536 to 2,318,030
_RS12765
Position (kb)
2314
2315
2316
2317 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2314.843 kb on - strand at 2314.892 kb on + strand at 2314.936 kb on - strand at 2315.091 kb on - strand, within SGL_RS12755 at 2315.153 kb on - strand, within SGL_RS12755 at 2315.174 kb on + strand, within SGL_RS12755 at 2315.258 kb on - strand, within SGL_RS12755 at 2315.469 kb on - strand, within SGL_RS12755 at 2315.588 kb on - strand, within SGL_RS12755 at 2315.681 kb on + strand, within SGL_RS12755 at 2315.681 kb on + strand, within SGL_RS12755 at 2315.689 kb on - strand, within SGL_RS12755 at 2315.742 kb on + strand, within SGL_RS12755 at 2316.275 kb on - strand, within SGL_RS12755 at 2316.285 kb on + strand, within SGL_RS12755 at 2316.287 kb on + strand, within SGL_RS12755 at 2316.411 kb on - strand, within SGL_RS12755 at 2316.432 kb on - strand, within SGL_RS12755 at 2316.468 kb on + strand, within SGL_RS12755 at 2316.676 kb on + strand, within SGL_RS12755 at 2316.676 kb on + strand, within SGL_RS12755 at 2316.684 kb on - strand, within SGL_RS12755 at 2316.684 kb on - strand, within SGL_RS12755 at 2316.684 kb on - strand, within SGL_RS12755 at 2316.694 kb on + strand, within SGL_RS12755 at 2316.696 kb on - strand, within SGL_RS12755 at 2316.708 kb on - strand, within SGL_RS12755 at 2316.724 kb on + strand at 2316.853 kb on - strand at 2316.867 kb on + strand at 2316.879 kb on + strand at 2316.933 kb on + strand at 2316.981 kb on + strand at 2316.981 kb on + strand at 2316.989 kb on - strand at 2317.032 kb on - strand at 2317.162 kb on - strand, within SGL_RS12760 at 2317.270 kb on - strand, within SGL_RS12760 at 2317.279 kb on + strand, within SGL_RS12760 at 2317.280 kb on - strand, within SGL_RS12760 at 2317.579 kb on - strand at 2317.609 kb on + strand, within SGL_RS12765 at 2317.628 kb on - strand, within SGL_RS12765 at 2317.724 kb on + strand, within SGL_RS12765 at 2317.769 kb on - strand, within SGL_RS12765 at 2317.824 kb on - strand, within SGL_RS12765 at 2317.833 kb on + strand, within SGL_RS12765 at 2317.833 kb on + strand, within SGL_RS12765
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 2,314,843 - +0.4 2,314,892 + -0.4 2,314,936 - -0.4 2,315,091 - SGL_RS12755 0.14 -0.5 2,315,153 - SGL_RS12755 0.17 +0.8 2,315,174 + SGL_RS12755 0.18 -0.4 2,315,258 - SGL_RS12755 0.22 -4.2 2,315,469 - SGL_RS12755 0.32 -1.8 2,315,588 - SGL_RS12755 0.38 -1.7 2,315,681 + SGL_RS12755 0.42 -2.5 2,315,681 + SGL_RS12755 0.42 -2.6 2,315,689 - SGL_RS12755 0.42 -2.0 2,315,742 + SGL_RS12755 0.45 -4.4 2,316,275 - SGL_RS12755 0.70 -1.1 2,316,285 + SGL_RS12755 0.70 -1.6 2,316,287 + SGL_RS12755 0.70 -1.1 2,316,411 - SGL_RS12755 0.76 -1.3 2,316,432 - SGL_RS12755 0.77 -2.1 2,316,468 + SGL_RS12755 0.79 -0.8 2,316,676 + SGL_RS12755 0.88 -1.6 2,316,676 + SGL_RS12755 0.88 -1.0 2,316,684 - SGL_RS12755 0.89 -1.4 2,316,684 - SGL_RS12755 0.89 -1.6 2,316,684 - SGL_RS12755 0.89 -1.4 2,316,694 + SGL_RS12755 0.89 -1.9 2,316,696 - SGL_RS12755 0.89 -1.2 2,316,708 - SGL_RS12755 0.90 -1.1 2,316,724 + -1.3 2,316,853 - -0.7 2,316,867 + -1.6 2,316,879 + -1.1 2,316,933 + -0.8 2,316,981 + -0.1 2,316,981 + +0.6 2,316,989 - -0.2 2,317,032 - +0.0 2,317,162 - SGL_RS12760 0.15 -0.0 2,317,270 - SGL_RS12760 0.47 +0.0 2,317,279 + SGL_RS12760 0.50 +1.0 2,317,280 - SGL_RS12760 0.50 +0.3 2,317,579 - -0.1 2,317,609 + SGL_RS12765 0.15 -0.1 2,317,628 - SGL_RS12765 0.19 +0.1 2,317,724 + SGL_RS12765 0.38 -0.3 2,317,769 - SGL_RS12765 0.47 +0.2 2,317,824 - SGL_RS12765 0.58 -0.2 2,317,833 + SGL_RS12765 0.60 +0.2 2,317,833 + SGL_RS12765 0.60 -0.3
Or see this region's nucleotide sequence