Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12575 and SGL_RS19930 are separated by 224 nucleotides SGL_RS19930 and SGL_RS12585 are separated by 67 nucleotides SGL_RS12585 and SGL_RS12590 are separated by 49 nucleotides SGL_RS12590 and SGL_RS12595 are separated by 88 nucleotides
SGL_RS12575: SGL_RS12575 - riboflavin synthase, at 2,280,267 to 2,280,947
_RS12575
SGL_RS19930: SGL_RS19930 - hypothetical protein, at 2,281,172 to 2,281,441
_RS19930
SGL_RS12585: SGL_RS12585 - ABC transporter permease subunit, at 2,281,509 to 2,283,182
_RS12585
SGL_RS12590: SGL_RS12590 - low molecular weight protein-tyrosine-phosphatase, at 2,283,232 to 2,283,705
_RS12590
SGL_RS12595: SGL_RS12595 - ROK family protein, at 2,283,794 to 2,284,777
_RS12595
Position (kb)
2281
2282
2283
2284 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2280.715 kb on + strand, within SGL_RS12575 at 2280.715 kb on + strand, within SGL_RS12575 at 2280.723 kb on - strand, within SGL_RS12575 at 2280.734 kb on - strand, within SGL_RS12575 at 2280.791 kb on + strand, within SGL_RS12575 at 2280.799 kb on - strand, within SGL_RS12575 at 2280.836 kb on + strand, within SGL_RS12575 at 2280.966 kb on - strand at 2281.039 kb on + strand at 2281.149 kb on - strand at 2281.156 kb on - strand at 2281.337 kb on + strand, within SGL_RS19930 at 2281.503 kb on - strand at 2281.548 kb on - strand at 2281.661 kb on + strand at 2281.699 kb on + strand, within SGL_RS12585 at 2281.840 kb on - strand, within SGL_RS12585 at 2281.850 kb on + strand, within SGL_RS12585 at 2281.887 kb on - strand, within SGL_RS12585 at 2281.931 kb on + strand, within SGL_RS12585 at 2282.104 kb on + strand, within SGL_RS12585 at 2282.111 kb on - strand, within SGL_RS12585 at 2282.364 kb on + strand, within SGL_RS12585 at 2282.397 kb on - strand, within SGL_RS12585 at 2282.487 kb on + strand, within SGL_RS12585 at 2282.601 kb on + strand, within SGL_RS12585 at 2282.781 kb on - strand, within SGL_RS12585 at 2282.932 kb on - strand, within SGL_RS12585 at 2282.942 kb on - strand, within SGL_RS12585 at 2283.606 kb on + strand, within SGL_RS12590 at 2283.621 kb on + strand, within SGL_RS12590 at 2283.629 kb on - strand, within SGL_RS12590 at 2283.808 kb on + strand at 2283.870 kb on + strand at 2283.994 kb on - strand, within SGL_RS12595 at 2284.018 kb on - strand, within SGL_RS12595 at 2284.089 kb on + strand, within SGL_RS12595
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 2,280,715 + SGL_RS12575 0.66 -3.3 2,280,715 + SGL_RS12575 0.66 -2.6 2,280,723 - SGL_RS12575 0.67 -3.0 2,280,734 - SGL_RS12575 0.69 -3.7 2,280,791 + SGL_RS12575 0.77 -3.6 2,280,799 - SGL_RS12575 0.78 -3.2 2,280,836 + SGL_RS12575 0.84 -2.3 2,280,966 - +0.0 2,281,039 + -0.2 2,281,149 - -1.0 2,281,156 - +0.0 2,281,337 + SGL_RS19930 0.61 -1.6 2,281,503 - -1.1 2,281,548 - -3.0 2,281,661 + +0.0 2,281,699 + SGL_RS12585 0.11 -2.3 2,281,840 - SGL_RS12585 0.20 -3.3 2,281,850 + SGL_RS12585 0.20 -3.2 2,281,887 - SGL_RS12585 0.23 -3.0 2,281,931 + SGL_RS12585 0.25 -3.2 2,282,104 + SGL_RS12585 0.36 -3.2 2,282,111 - SGL_RS12585 0.36 -2.6 2,282,364 + SGL_RS12585 0.51 -3.3 2,282,397 - SGL_RS12585 0.53 -2.0 2,282,487 + SGL_RS12585 0.58 +0.0 2,282,601 + SGL_RS12585 0.65 -2.6 2,282,781 - SGL_RS12585 0.76 -4.1 2,282,932 - SGL_RS12585 0.85 -3.2 2,282,942 - SGL_RS12585 0.86 -2.6 2,283,606 + SGL_RS12590 0.79 +2.0 2,283,621 + SGL_RS12590 0.82 +0.4 2,283,629 - SGL_RS12590 0.84 +0.2 2,283,808 + +0.4 2,283,870 + +0.3 2,283,994 - SGL_RS12595 0.20 +0.0 2,284,018 - SGL_RS12595 0.23 -0.1 2,284,089 + SGL_RS12595 0.30 -0.2
Or see this region's nucleotide sequence