Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12330 and SGL_RS19240 are separated by 249 nucleotides SGL_RS19240 and SGL_RS12335 are separated by 76 nucleotides SGL_RS12335 and SGL_RS12340 are separated by 67 nucleotides
SGL_RS12330: SGL_RS12330 - IS5 family transposase, at 2,226,619 to 2,227,404
_RS12330
SGL_RS19240: SGL_RS19240 - ABC transporter ATP-binding protein, at 2,227,654 to 2,227,752
_RS19240
SGL_RS12335: SGL_RS12335 - dTDP-4-dehydrorhamnose 3,5-epimerase, at 2,227,829 to 2,228,377
_RS12335
SGL_RS12340: SGL_RS12340 - hypothetical protein, at 2,228,445 to 2,230,328
_RS12340
Position (kb)
2227
2228
2229 Strain fitness (log2 ratio)
-2
-1
0
1 at 2227.547 kb on - strand at 2227.609 kb on - strand at 2227.816 kb on - strand at 2228.171 kb on + strand, within SGL_RS12335 at 2228.205 kb on + strand, within SGL_RS12335 at 2228.213 kb on + strand, within SGL_RS12335 at 2228.213 kb on + strand, within SGL_RS12335 at 2228.218 kb on + strand, within SGL_RS12335 at 2228.218 kb on + strand, within SGL_RS12335 at 2228.221 kb on - strand, within SGL_RS12335 at 2228.243 kb on - strand, within SGL_RS12335 at 2228.247 kb on + strand, within SGL_RS12335 at 2228.271 kb on - strand, within SGL_RS12335 at 2228.431 kb on + strand at 2228.473 kb on - strand at 2228.538 kb on + strand at 2228.715 kb on + strand, within SGL_RS12340 at 2228.755 kb on + strand, within SGL_RS12340 at 2228.815 kb on + strand, within SGL_RS12340 at 2228.857 kb on - strand, within SGL_RS12340 at 2228.952 kb on + strand, within SGL_RS12340 at 2229.190 kb on - strand, within SGL_RS12340 at 2229.216 kb on + strand, within SGL_RS12340
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 2,227,547 - +0.3 2,227,609 - +0.2 2,227,816 - +0.6 2,228,171 + SGL_RS12335 0.62 -1.4 2,228,205 + SGL_RS12335 0.68 -2.5 2,228,213 + SGL_RS12335 0.70 -1.3 2,228,213 + SGL_RS12335 0.70 -1.2 2,228,218 + SGL_RS12335 0.71 -1.8 2,228,218 + SGL_RS12335 0.71 -1.4 2,228,221 - SGL_RS12335 0.71 -1.5 2,228,243 - SGL_RS12335 0.75 -0.2 2,228,247 + SGL_RS12335 0.76 -1.2 2,228,271 - SGL_RS12335 0.81 -2.1 2,228,431 + -0.3 2,228,473 - +0.2 2,228,538 + -1.8 2,228,715 + SGL_RS12340 0.14 -1.8 2,228,755 + SGL_RS12340 0.16 -0.2 2,228,815 + SGL_RS12340 0.20 -1.3 2,228,857 - SGL_RS12340 0.22 -0.8 2,228,952 + SGL_RS12340 0.27 -1.2 2,229,190 - SGL_RS12340 0.40 -0.5 2,229,216 + SGL_RS12340 0.41 -0.7
Or see this region's nucleotide sequence