Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11125 and SGL_RS11130 are separated by 107 nucleotides SGL_RS11130 and SGL_RS11135 are separated by 78 nucleotides SGL_RS11135 and SGL_RS11140 are separated by 110 nucleotides
SGL_RS11125: SGL_RS11125 - 50S ribosomal protein L21, at 1,957,758 to 1,958,132
_RS11125
SGL_RS11130: SGL_RS11130 - 50S ribosomal protein L27, at 1,958,240 to 1,958,503
_RS11130
SGL_RS11135: SGL_RS11135 - D-alanyl-D-alanine dipeptidase DdpX, at 1,958,582 to 1,959,325
_RS11135
SGL_RS11140: SGL_RS11140 - citramalate synthase, at 1,959,436 to 1,961,079
_RS11140
Position (kb)
1958
1959
1960 Strain fitness (log2 ratio)
-2
-1
0
1 at 1958.127 kb on + strand at 1958.686 kb on - strand, within SGL_RS11135 at 1958.849 kb on - strand, within SGL_RS11135 at 1959.290 kb on - strand at 1959.546 kb on + strand at 1959.738 kb on - strand, within SGL_RS11140 at 1959.792 kb on - strand, within SGL_RS11140 at 1959.792 kb on - strand, within SGL_RS11140 at 1959.840 kb on - strand, within SGL_RS11140 at 1959.845 kb on - strand, within SGL_RS11140 at 1959.878 kb on + strand, within SGL_RS11140 at 1959.908 kb on - strand, within SGL_RS11140 at 1959.994 kb on + strand, within SGL_RS11140 at 1959.994 kb on + strand, within SGL_RS11140 at 1959.994 kb on + strand, within SGL_RS11140 at 1960.059 kb on - strand, within SGL_RS11140 at 1960.120 kb on + strand, within SGL_RS11140 at 1960.158 kb on - strand, within SGL_RS11140 at 1960.260 kb on - strand, within SGL_RS11140
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,958,127 + -0.5 1,958,686 - SGL_RS11135 0.14 -2.1 1,958,849 - SGL_RS11135 0.36 +0.1 1,959,290 - -1.4 1,959,546 + +0.1 1,959,738 - SGL_RS11140 0.18 +0.3 1,959,792 - SGL_RS11140 0.22 -0.5 1,959,792 - SGL_RS11140 0.22 -0.1 1,959,840 - SGL_RS11140 0.25 -0.2 1,959,845 - SGL_RS11140 0.25 +0.2 1,959,878 + SGL_RS11140 0.27 +0.4 1,959,908 - SGL_RS11140 0.29 -0.1 1,959,994 + SGL_RS11140 0.34 +0.2 1,959,994 + SGL_RS11140 0.34 +0.3 1,959,994 + SGL_RS11140 0.34 +0.1 1,960,059 - SGL_RS11140 0.38 -0.1 1,960,120 + SGL_RS11140 0.42 -0.0 1,960,158 - SGL_RS11140 0.44 +0.8 1,960,260 - SGL_RS11140 0.50 +0.1
Or see this region's nucleotide sequence