Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11020 and SGL_RS11025 are separated by 381 nucleotides SGL_RS11025 and SGL_RS11030 are separated by 16 nucleotides SGL_RS11030 and SGL_RS11035 are separated by 76 nucleotides
SGL_RS11020: SGL_RS11020 - geranylgeranyl reductase, at 1,934,307 to 1,935,530
_RS11020
SGL_RS11025: SGL_RS11025 - oxidoreductase, at 1,935,912 to 1,937,069
_RS11025
SGL_RS11030: SGL_RS11030 - DUF433 domain-containing protein, at 1,937,086 to 1,937,397
_RS11030
SGL_RS11035: SGL_RS11035 - LapA family protein, at 1,937,474 to 1,938,310
_RS11035
Position (kb) 1935
1936
1937
1938 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 1935.607 kb on + strand at 1935.698 kb on - strand at 1935.698 kb on - strand at 1935.832 kb on + strand at 1935.895 kb on - strand at 1936.031 kb on + strand, within SGL_RS11025 at 1936.124 kb on - strand, within SGL_RS11025 at 1936.232 kb on - strand, within SGL_RS11025 at 1936.362 kb on + strand, within SGL_RS11025 at 1936.362 kb on + strand, within SGL_RS11025 at 1936.370 kb on - strand, within SGL_RS11025 at 1936.370 kb on - strand, within SGL_RS11025 at 1936.370 kb on - strand, within SGL_RS11025 at 1936.621 kb on - strand, within SGL_RS11025 at 1936.655 kb on - strand, within SGL_RS11025 at 1936.725 kb on - strand, within SGL_RS11025 at 1936.815 kb on + strand, within SGL_RS11025 at 1937.474 kb on + strand at 1937.641 kb on + strand, within SGL_RS11035 at 1937.902 kb on - strand, within SGL_RS11035 at 1937.978 kb on + strand, within SGL_RS11035
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,935,607 + -0.1 1,935,698 - -0.1 1,935,698 - -0.4 1,935,832 + -0.2 1,935,895 - -0.0 1,936,031 + SGL_RS11025 0.10 -2.5 1,936,124 - SGL_RS11025 0.18 -3.6 1,936,232 - SGL_RS11025 0.28 -2.2 1,936,362 + SGL_RS11025 0.39 -4.2 1,936,362 + SGL_RS11025 0.39 -1.6 1,936,370 - SGL_RS11025 0.40 -1.9 1,936,370 - SGL_RS11025 0.40 -2.4 1,936,370 - SGL_RS11025 0.40 -2.9 1,936,621 - SGL_RS11025 0.61 -2.2 1,936,655 - SGL_RS11025 0.64 -1.6 1,936,725 - SGL_RS11025 0.70 -5.3 1,936,815 + SGL_RS11025 0.78 -2.9 1,937,474 + -0.3 1,937,641 + SGL_RS11035 0.20 -3.5 1,937,902 - SGL_RS11035 0.51 -0.6 1,937,978 + SGL_RS11035 0.60 +0.2
Or see this region's nucleotide sequence