Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10995 and SGL_RS11000 are separated by 512 nucleotides SGL_RS11000 and SGL_RS11005 are separated by 42 nucleotides
SGL_RS10995: SGL_RS10995 - ribonucleoside-diphosphate reductase subunit alpha, at 1,928,323 to 1,930,626
_RS10995
SGL_RS11000: SGL_RS11000 - sulfate adenylyltransferase, at 1,931,139 to 1,932,311
_RS11000
SGL_RS11005: SGL_RS11005 - glycosyltransferase, at 1,932,354 to 1,933,418
_RS11005
Position (kb)
1931
1932
1933 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1930.707 kb on - strand at 1930.709 kb on - strand at 1930.931 kb on - strand at 1931.136 kb on + strand at 1931.310 kb on - strand, within SGL_RS11000 at 1931.312 kb on + strand, within SGL_RS11000 at 1931.439 kb on - strand, within SGL_RS11000 at 1931.446 kb on + strand, within SGL_RS11000 at 1931.584 kb on + strand, within SGL_RS11000 at 1931.607 kb on - strand, within SGL_RS11000 at 1931.734 kb on + strand, within SGL_RS11000 at 1931.757 kb on + strand, within SGL_RS11000 at 1931.819 kb on - strand, within SGL_RS11000 at 1931.936 kb on + strand, within SGL_RS11000 at 1931.944 kb on - strand, within SGL_RS11000 at 1932.030 kb on + strand, within SGL_RS11000 at 1932.186 kb on - strand, within SGL_RS11000 at 1932.186 kb on - strand, within SGL_RS11000 at 1932.267 kb on + strand at 1932.404 kb on + strand at 1932.716 kb on - strand, within SGL_RS11005 at 1932.744 kb on + strand, within SGL_RS11005 at 1932.752 kb on - strand, within SGL_RS11005 at 1932.771 kb on + strand, within SGL_RS11005 at 1932.816 kb on + strand, within SGL_RS11005 at 1932.823 kb on + strand, within SGL_RS11005 at 1932.868 kb on + strand, within SGL_RS11005 at 1933.055 kb on - strand, within SGL_RS11005 at 1933.122 kb on + strand, within SGL_RS11005 at 1933.224 kb on - strand, within SGL_RS11005
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,930,707 - +0.1 1,930,709 - -0.2 1,930,931 - +0.1 1,931,136 + +1.0 1,931,310 - SGL_RS11000 0.15 -3.1 1,931,312 + SGL_RS11000 0.15 -3.8 1,931,439 - SGL_RS11000 0.26 -4.0 1,931,446 + SGL_RS11000 0.26 -4.6 1,931,584 + SGL_RS11000 0.38 -2.9 1,931,607 - SGL_RS11000 0.40 -5.3 1,931,734 + SGL_RS11000 0.51 -4.2 1,931,757 + SGL_RS11000 0.53 -2.9 1,931,819 - SGL_RS11000 0.58 -4.1 1,931,936 + SGL_RS11000 0.68 -3.5 1,931,944 - SGL_RS11000 0.69 -3.5 1,932,030 + SGL_RS11000 0.76 -3.5 1,932,186 - SGL_RS11000 0.89 -3.2 1,932,186 - SGL_RS11000 0.89 -4.4 1,932,267 + -4.5 1,932,404 + -0.3 1,932,716 - SGL_RS11005 0.34 -0.7 1,932,744 + SGL_RS11005 0.37 -0.4 1,932,752 - SGL_RS11005 0.37 -0.6 1,932,771 + SGL_RS11005 0.39 -0.3 1,932,816 + SGL_RS11005 0.43 -0.6 1,932,823 + SGL_RS11005 0.44 -0.2 1,932,868 + SGL_RS11005 0.48 -0.4 1,933,055 - SGL_RS11005 0.66 -0.8 1,933,122 + SGL_RS11005 0.72 -1.1 1,933,224 - SGL_RS11005 0.82 -0.6
Or see this region's nucleotide sequence