Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10315 and SGL_RS10320 are separated by 192 nucleotides SGL_RS10320 and SGL_RS10325 are separated by 326 nucleotides
SGL_RS10315: SGL_RS10315 - hypothetical protein, at 1,785,835 to 1,788,357
_RS10315
SGL_RS10320: SGL_RS10320 - ribosome biogenesis GTPase Der, at 1,788,550 to 1,789,908
_RS10320
SGL_RS10325: SGL_RS10325 - hypothetical protein, at 1,790,235 to 1,791,353
_RS10325
Position (kb)
1788
1789
1790 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1787.647 kb on + strand, within SGL_RS10315 at 1787.675 kb on - strand, within SGL_RS10315 at 1787.776 kb on - strand, within SGL_RS10315 at 1787.847 kb on + strand, within SGL_RS10315 at 1787.871 kb on - strand, within SGL_RS10315 at 1788.071 kb on - strand, within SGL_RS10315 at 1788.071 kb on - strand, within SGL_RS10315 at 1788.144 kb on - strand at 1788.202 kb on + strand at 1788.202 kb on + strand at 1788.289 kb on + strand at 1788.296 kb on + strand at 1789.021 kb on + strand, within SGL_RS10320 at 1789.100 kb on + strand, within SGL_RS10320 at 1789.178 kb on + strand, within SGL_RS10320 at 1789.186 kb on - strand, within SGL_RS10320 at 1789.369 kb on - strand, within SGL_RS10320 at 1789.550 kb on + strand, within SGL_RS10320 at 1789.972 kb on + strand at 1790.038 kb on - strand at 1790.232 kb on + strand at 1790.253 kb on - strand at 1790.293 kb on + strand at 1790.538 kb on + strand, within SGL_RS10325 at 1790.639 kb on - strand, within SGL_RS10325 at 1790.745 kb on + strand, within SGL_RS10325 at 1790.846 kb on + strand, within SGL_RS10325 at 1790.874 kb on + strand, within SGL_RS10325 at 1790.889 kb on - strand, within SGL_RS10325
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,787,647 + SGL_RS10315 0.72 +0.0 1,787,675 - SGL_RS10315 0.73 -0.8 1,787,776 - SGL_RS10315 0.77 +0.1 1,787,847 + SGL_RS10315 0.80 -0.6 1,787,871 - SGL_RS10315 0.81 +0.2 1,788,071 - SGL_RS10315 0.89 -0.8 1,788,071 - SGL_RS10315 0.89 +0.1 1,788,144 - +0.4 1,788,202 + +0.2 1,788,202 + +0.0 1,788,289 + +0.6 1,788,296 + +0.1 1,789,021 + SGL_RS10320 0.35 -2.4 1,789,100 + SGL_RS10320 0.40 -3.7 1,789,178 + SGL_RS10320 0.46 -2.4 1,789,186 - SGL_RS10320 0.47 -1.1 1,789,369 - SGL_RS10320 0.60 -1.2 1,789,550 + SGL_RS10320 0.74 -3.4 1,789,972 + +0.2 1,790,038 - +0.0 1,790,232 + -0.0 1,790,253 - +0.2 1,790,293 + -0.1 1,790,538 + SGL_RS10325 0.27 +0.0 1,790,639 - SGL_RS10325 0.36 -0.1 1,790,745 + SGL_RS10325 0.46 -0.0 1,790,846 + SGL_RS10325 0.55 +0.6 1,790,874 + SGL_RS10325 0.57 -0.6 1,790,889 - SGL_RS10325 0.58 +0.1
Or see this region's nucleotide sequence