Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10145 and SGL_RS10150 are separated by 243 nucleotides SGL_RS10150 and SGL_RS10155 are separated by 181 nucleotides SGL_RS10155 and SGL_RS10160 are separated by 64 nucleotides
SGL_RS10145: SGL_RS10145 - cytochrome c, at 1,750,681 to 1,751,067
_RS10145
SGL_RS10150: SGL_RS10150 - 50S ribosomal protein L9, at 1,751,311 to 1,751,769
_RS10150
SGL_RS10155: SGL_RS10155 - hypothetical protein, at 1,751,951 to 1,752,826
_RS10155
SGL_RS10160: SGL_RS10160 - B12-binding domain-containing radical SAM protein, at 1,752,891 to 1,754,501
_RS10160
Position (kb)
1751
1752
1753 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1751.070 kb on - strand at 1751.070 kb on - strand at 1751.175 kb on - strand at 1751.408 kb on + strand, within SGL_RS10150 at 1751.478 kb on - strand, within SGL_RS10150 at 1751.649 kb on + strand, within SGL_RS10150 at 1751.936 kb on + strand at 1752.017 kb on - strand at 1752.045 kb on - strand, within SGL_RS10155 at 1752.330 kb on + strand, within SGL_RS10155 at 1752.424 kb on - strand, within SGL_RS10155 at 1752.435 kb on + strand, within SGL_RS10155 at 1752.451 kb on + strand, within SGL_RS10155 at 1752.461 kb on - strand, within SGL_RS10155 at 1752.461 kb on - strand, within SGL_RS10155 at 1752.692 kb on + strand, within SGL_RS10155 at 1752.710 kb on - strand, within SGL_RS10155 at 1752.948 kb on + strand at 1752.963 kb on - strand at 1753.034 kb on - strand at 1753.424 kb on - strand, within SGL_RS10160 at 1753.426 kb on + strand, within SGL_RS10160 at 1753.434 kb on - strand, within SGL_RS10160 at 1753.439 kb on - strand, within SGL_RS10160 at 1753.479 kb on - strand, within SGL_RS10160
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,751,070 - +0.0 1,751,070 - +0.0 1,751,175 - -0.6 1,751,408 + SGL_RS10150 0.21 -0.0 1,751,478 - SGL_RS10150 0.36 +0.1 1,751,649 + SGL_RS10150 0.74 +0.1 1,751,936 + -1.1 1,752,017 - -2.3 1,752,045 - SGL_RS10155 0.11 -0.1 1,752,330 + SGL_RS10155 0.43 -0.1 1,752,424 - SGL_RS10155 0.54 -0.5 1,752,435 + SGL_RS10155 0.55 -2.4 1,752,451 + SGL_RS10155 0.57 -2.1 1,752,461 - SGL_RS10155 0.58 -3.3 1,752,461 - SGL_RS10155 0.58 -4.0 1,752,692 + SGL_RS10155 0.85 -2.4 1,752,710 - SGL_RS10155 0.87 -1.9 1,752,948 + +0.6 1,752,963 - -0.7 1,753,034 - +1.0 1,753,424 - SGL_RS10160 0.33 -0.8 1,753,426 + SGL_RS10160 0.33 -0.5 1,753,434 - SGL_RS10160 0.34 -0.8 1,753,439 - SGL_RS10160 0.34 -0.0 1,753,479 - SGL_RS10160 0.36 -0.4
Or see this region's nucleotide sequence