Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS10125 and SGL_RS10130 are separated by 214 nucleotides SGL_RS10130 and SGL_RS10135 are separated by 156 nucleotides
SGL_RS10125: SGL_RS10125 - glucose-6-phosphate isomerase, at 1,744,498 to 1,746,093
_RS10125
SGL_RS10130: SGL_RS10130 - fatty acid desaturase, at 1,746,308 to 1,747,363
_RS10130
SGL_RS10135: SGL_RS10135 - hypothetical protein, at 1,747,520 to 1,748,893
_RS10135
Position (kb)
1746
1747
1748 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1745.514 kb on + strand, within SGL_RS10125 at 1746.300 kb on - strand at 1746.396 kb on + strand at 1746.452 kb on - strand, within SGL_RS10130 at 1746.462 kb on + strand, within SGL_RS10130 at 1746.506 kb on + strand, within SGL_RS10130 at 1746.548 kb on + strand, within SGL_RS10130 at 1746.581 kb on - strand, within SGL_RS10130 at 1746.652 kb on + strand, within SGL_RS10130 at 1746.652 kb on + strand, within SGL_RS10130 at 1746.806 kb on + strand, within SGL_RS10130 at 1746.840 kb on + strand, within SGL_RS10130 at 1746.840 kb on + strand, within SGL_RS10130 at 1746.853 kb on - strand, within SGL_RS10130 at 1746.931 kb on + strand, within SGL_RS10130 at 1746.961 kb on + strand, within SGL_RS10130 at 1746.962 kb on - strand, within SGL_RS10130 at 1747.031 kb on + strand, within SGL_RS10130 at 1747.107 kb on - strand, within SGL_RS10130 at 1747.142 kb on - strand, within SGL_RS10130 at 1747.308 kb on + strand at 1747.655 kb on + strand at 1747.825 kb on + strand, within SGL_RS10135 at 1748.051 kb on - strand, within SGL_RS10135 at 1748.135 kb on - strand, within SGL_RS10135 at 1748.156 kb on - strand, within SGL_RS10135 at 1748.311 kb on + strand, within SGL_RS10135 at 1748.311 kb on + strand, within SGL_RS10135 at 1748.316 kb on + strand, within SGL_RS10135 at 1748.326 kb on + strand, within SGL_RS10135 at 1748.334 kb on - strand, within SGL_RS10135 at 1748.334 kb on - strand, within SGL_RS10135
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,745,514 + SGL_RS10125 0.64 -1.6 1,746,300 - -2.9 1,746,396 + -2.9 1,746,452 - SGL_RS10130 0.14 -1.8 1,746,462 + SGL_RS10130 0.15 -3.6 1,746,506 + SGL_RS10130 0.19 -2.3 1,746,548 + SGL_RS10130 0.23 -2.5 1,746,581 - SGL_RS10130 0.26 -0.5 1,746,652 + SGL_RS10130 0.33 -2.5 1,746,652 + SGL_RS10130 0.33 -2.0 1,746,806 + SGL_RS10130 0.47 -2.1 1,746,840 + SGL_RS10130 0.50 -2.8 1,746,840 + SGL_RS10130 0.50 -2.2 1,746,853 - SGL_RS10130 0.52 -2.8 1,746,931 + SGL_RS10130 0.59 -2.0 1,746,961 + SGL_RS10130 0.62 -2.8 1,746,962 - SGL_RS10130 0.62 -2.4 1,747,031 + SGL_RS10130 0.68 -3.0 1,747,107 - SGL_RS10130 0.76 -2.5 1,747,142 - SGL_RS10130 0.79 -2.5 1,747,308 + -2.9 1,747,655 + -0.1 1,747,825 + SGL_RS10135 0.22 +0.3 1,748,051 - SGL_RS10135 0.39 -0.3 1,748,135 - SGL_RS10135 0.45 +0.2 1,748,156 - SGL_RS10135 0.46 -0.1 1,748,311 + SGL_RS10135 0.58 -0.3 1,748,311 + SGL_RS10135 0.58 +0.4 1,748,316 + SGL_RS10135 0.58 -0.2 1,748,326 + SGL_RS10135 0.59 -0.1 1,748,334 - SGL_RS10135 0.59 +0.2 1,748,334 - SGL_RS10135 0.59 +0.5
Or see this region's nucleotide sequence