Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09800 and SGL_RS09805 are separated by 107 nucleotides SGL_RS09805 and SGL_RS09810 are separated by 107 nucleotides SGL_RS09810 and SGL_RS09815 are separated by 159 nucleotides
SGL_RS09800: SGL_RS09800 - FAD-binding oxidoreductase, at 1,685,899 to 1,687,218
_RS09800
SGL_RS09805: SGL_RS09805 - photosystem I reaction center subunit IX, at 1,687,326 to 1,687,448
_RS09805
SGL_RS09810: SGL_RS09810 - photosystem I reaction center subunit III, at 1,687,556 to 1,688,053
_RS09810
SGL_RS09815: SGL_RS09815 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 1,688,213 to 1,689,259
_RS09815
Position (kb)
1687
1688 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1686.406 kb on + strand, within SGL_RS09800 at 1686.421 kb on + strand, within SGL_RS09800 at 1686.429 kb on - strand, within SGL_RS09800 at 1686.544 kb on - strand, within SGL_RS09800 at 1686.862 kb on + strand, within SGL_RS09800 at 1687.504 kb on - strand at 1687.831 kb on + strand, within SGL_RS09810 at 1688.024 kb on + strand at 1688.025 kb on - strand at 1688.370 kb on - strand, within SGL_RS09815 at 1688.393 kb on - strand, within SGL_RS09815 at 1688.419 kb on - strand, within SGL_RS09815 at 1688.419 kb on - strand, within SGL_RS09815
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,686,406 + SGL_RS09800 0.38 -1.2 1,686,421 + SGL_RS09800 0.40 -0.3 1,686,429 - SGL_RS09800 0.40 +1.9 1,686,544 - SGL_RS09800 0.49 +1.0 1,686,862 + SGL_RS09800 0.73 -0.5 1,687,504 - -0.3 1,687,831 + SGL_RS09810 0.55 -0.7 1,688,024 + -2.9 1,688,025 - -0.6 1,688,370 - SGL_RS09815 0.15 -0.3 1,688,393 - SGL_RS09815 0.17 -0.2 1,688,419 - SGL_RS09815 0.20 -0.8 1,688,419 - SGL_RS09815 0.20 -0.0
Or see this region's nucleotide sequence