Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08750 and SGL_RS08755 are separated by 45 nucleotides SGL_RS08755 and SGL_RS08760 are separated by 40 nucleotides SGL_RS08760 and SGL_RS08765 are separated by 42 nucleotides SGL_RS08765 and SGL_RS08770 are separated by 78 nucleotides SGL_RS08770 and SGL_RS08775 are separated by 129 nucleotides
SGL_RS08750: SGL_RS08750 - YdcF family protein, at 1,469,013 to 1,469,801
_RS08750
SGL_RS08755: SGL_RS08755 - 3-hydroxyacyl-ACP dehydratase FabZ, at 1,469,847 to 1,470,347
_RS08755
SGL_RS08760: SGL_RS08760 - hypothetical protein, at 1,470,388 to 1,470,567
_RS08760
SGL_RS08765: SGL_RS08765 - 2-phosphosulfolactate phosphatase family protein, at 1,470,610 to 1,471,335
_RS08765
SGL_RS08770: SGL_RS08770 - nitroreductase family protein, at 1,471,414 to 1,472,019
_RS08770
SGL_RS08775: SGL_RS08775 - aspartate--tRNA ligase, at 1,472,149 to 1,473,948
_RS08775
Position (kb) 1470
1471
1472 Strain fitness (log2 ratio) -2
-1
0
1 at 1469.615 kb on - strand, within SGL_RS08750 at 1469.638 kb on - strand, within SGL_RS08750 at 1470.483 kb on + strand, within SGL_RS08760 at 1470.941 kb on + strand, within SGL_RS08765 at 1470.950 kb on - strand, within SGL_RS08765 at 1470.992 kb on - strand, within SGL_RS08765 at 1471.080 kb on + strand, within SGL_RS08765
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,469,615 - SGL_RS08750 0.76 +0.3 1,469,638 - SGL_RS08750 0.79 +0.1 1,470,483 + SGL_RS08760 0.53 +0.3 1,470,941 + SGL_RS08765 0.46 +0.7 1,470,950 - SGL_RS08765 0.47 -1.1 1,470,992 - SGL_RS08765 0.53 -2.0 1,471,080 + SGL_RS08765 0.65 -0.7
Or see this region's nucleotide sequence