Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07830 and SGL_RS07835 are separated by 90 nucleotides SGL_RS07835 and SGL_RS07840 are separated by 78 nucleotides SGL_RS07840 and SGL_RS07845 are separated by 125 nucleotides SGL_RS07845 and SGL_RS07850 are separated by 49 nucleotides
SGL_RS07830: SGL_RS07830 - DNA adenine methylase, at 1,274,352 to 1,275,281
_RS07830
SGL_RS07835: SGL_RS07835 - IS5 family transposase, at 1,275,372 to 1,276,157
_RS07835
SGL_RS07840: SGL_RS07840 - SDR family NAD(P)-dependent oxidoreductase, at 1,276,236 to 1,276,982
_RS07840
SGL_RS07845: SGL_RS07845 - DUF427 domain-containing protein, at 1,277,108 to 1,277,392
_RS07845
SGL_RS07850: SGL_RS07850 - response regulator transcription factor, at 1,277,442 to 1,278,074
_RS07850
Position (kb)
1276
1277 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1275.874 kb on + strand, within SGL_RS07835 at 1276.602 kb on + strand, within SGL_RS07840 at 1276.609 kb on + strand, within SGL_RS07840 at 1276.619 kb on + strand, within SGL_RS07840 at 1276.627 kb on - strand, within SGL_RS07840 at 1276.801 kb on + strand, within SGL_RS07840 at 1276.934 kb on + strand at 1276.936 kb on - strand at 1276.942 kb on - strand at 1277.127 kb on - strand at 1277.168 kb on + strand, within SGL_RS07845 at 1277.169 kb on + strand, within SGL_RS07845 at 1277.181 kb on + strand, within SGL_RS07845 at 1277.189 kb on - strand, within SGL_RS07845 at 1277.189 kb on - strand, within SGL_RS07845 at 1277.345 kb on - strand, within SGL_RS07845 at 1277.401 kb on - strand at 1277.402 kb on - strand at 1277.421 kb on + strand at 1277.422 kb on + strand at 1277.710 kb on - strand, within SGL_RS07850 at 1277.721 kb on - strand, within SGL_RS07850 at 1277.750 kb on + strand, within SGL_RS07850 at 1277.759 kb on - strand, within SGL_RS07850 at 1277.801 kb on - strand, within SGL_RS07850 at 1277.930 kb on + strand, within SGL_RS07850
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,275,874 + SGL_RS07835 0.64 +0.6 1,276,602 + SGL_RS07840 0.49 -1.1 1,276,609 + SGL_RS07840 0.50 -4.2 1,276,619 + SGL_RS07840 0.51 -4.1 1,276,627 - SGL_RS07840 0.52 -3.2 1,276,801 + SGL_RS07840 0.76 -4.3 1,276,934 + -1.6 1,276,936 - -2.1 1,276,942 - -2.1 1,277,127 - -0.1 1,277,168 + SGL_RS07845 0.21 +0.1 1,277,169 + SGL_RS07845 0.21 -0.3 1,277,181 + SGL_RS07845 0.26 +0.1 1,277,189 - SGL_RS07845 0.28 -0.2 1,277,189 - SGL_RS07845 0.28 +0.3 1,277,345 - SGL_RS07845 0.83 +0.1 1,277,401 - -0.8 1,277,402 - +0.5 1,277,421 + +0.0 1,277,422 + -0.1 1,277,710 - SGL_RS07850 0.42 +0.1 1,277,721 - SGL_RS07850 0.44 -0.1 1,277,750 + SGL_RS07850 0.49 -0.2 1,277,759 - SGL_RS07850 0.50 +1.2 1,277,801 - SGL_RS07850 0.57 +0.3 1,277,930 + SGL_RS07850 0.77 -0.1
Or see this region's nucleotide sequence