Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06625 and SGL_RS06630 are separated by 47 nucleotides SGL_RS06630 and SGL_RS06635 are separated by 104 nucleotides SGL_RS06635 and SGL_RS06640 are separated by 156 nucleotides SGL_RS06640 and SGL_RS06645 are separated by 28 nucleotides
SGL_RS06625: SGL_RS06625 - S41 family peptidase, at 1,015,511 to 1,016,782
_RS06625
SGL_RS06630: SGL_RS06630 - Uma2 family endonuclease, at 1,016,830 to 1,017,495
_RS06630
SGL_RS06635: SGL_RS06635 - 16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH, at 1,017,600 to 1,018,493
_RS06635
SGL_RS06640: SGL_RS06640 - DUF4168 domain-containing protein, at 1,018,650 to 1,019,126
_RS06640
SGL_RS06645: SGL_RS06645 - urease accessory protein UreE, at 1,019,155 to 1,019,583
_RS06645
Position (kb) 1017
1018
1019 Strain fitness (log2 ratio) -1
0
1 at 1016.842 kb on + strand at 1017.079 kb on + strand, within SGL_RS06630 at 1017.089 kb on + strand, within SGL_RS06630 at 1017.158 kb on - strand, within SGL_RS06630 at 1017.158 kb on - strand, within SGL_RS06630 at 1017.364 kb on - strand, within SGL_RS06630 at 1017.416 kb on + strand, within SGL_RS06630 at 1017.490 kb on - strand at 1018.165 kb on + strand, within SGL_RS06635 at 1018.315 kb on - strand, within SGL_RS06635 at 1018.326 kb on - strand, within SGL_RS06635 at 1018.460 kb on - strand at 1018.463 kb on + strand at 1018.503 kb on + strand at 1018.555 kb on - strand at 1018.653 kb on - strand at 1018.848 kb on + strand, within SGL_RS06640 at 1018.915 kb on + strand, within SGL_RS06640 at 1018.955 kb on - strand, within SGL_RS06640 at 1019.332 kb on + strand, within SGL_RS06645 at 1019.372 kb on - strand, within SGL_RS06645
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,016,842 + +0.1 1,017,079 + SGL_RS06630 0.37 +0.2 1,017,089 + SGL_RS06630 0.39 +0.0 1,017,158 - SGL_RS06630 0.49 +0.0 1,017,158 - SGL_RS06630 0.49 -0.0 1,017,364 - SGL_RS06630 0.80 +0.0 1,017,416 + SGL_RS06630 0.88 -0.0 1,017,490 - -0.1 1,018,165 + SGL_RS06635 0.63 -1.4 1,018,315 - SGL_RS06635 0.80 -1.6 1,018,326 - SGL_RS06635 0.81 -0.9 1,018,460 - -0.5 1,018,463 + -1.2 1,018,503 + +1.0 1,018,555 - +0.1 1,018,653 - +0.7 1,018,848 + SGL_RS06640 0.42 +1.2 1,018,915 + SGL_RS06640 0.56 -1.7 1,018,955 - SGL_RS06640 0.64 -0.8 1,019,332 + SGL_RS06645 0.41 +0.2 1,019,372 - SGL_RS06645 0.51 +0.1
Or see this region's nucleotide sequence