Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS06585 and SGL_RS06590 are separated by 74 nucleotides SGL_RS06590 and SGL_RS06595 are separated by 85 nucleotides
SGL_RS06585: SGL_RS06585 - nitrate ABC transporter ATP-binding protein, at 1,006,234 to 1,008,246
_RS06585
SGL_RS06590: SGL_RS06590 - nitrate ABC transporter permease, at 1,008,321 to 1,009,148
_RS06590
SGL_RS06595: SGL_RS06595 - CmpA/NrtA family ABC transporter substrate-binding protein, at 1,009,234 to 1,010,574
_RS06595
Position (kb)
1008
1009
1010 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1007.660 kb on - strand, within SGL_RS06585 at 1007.864 kb on + strand, within SGL_RS06585 at 1007.869 kb on + strand, within SGL_RS06585 at 1008.187 kb on - strand at 1008.438 kb on + strand, within SGL_RS06590 at 1008.438 kb on + strand, within SGL_RS06590 at 1008.647 kb on - strand, within SGL_RS06590 at 1008.724 kb on - strand, within SGL_RS06590 at 1008.756 kb on + strand, within SGL_RS06590 at 1008.842 kb on + strand, within SGL_RS06590 at 1008.866 kb on + strand, within SGL_RS06590 at 1008.886 kb on - strand, within SGL_RS06590 at 1008.991 kb on + strand, within SGL_RS06590 at 1008.999 kb on - strand, within SGL_RS06590 at 1009.002 kb on + strand, within SGL_RS06590 at 1009.200 kb on - strand at 1009.272 kb on - strand at 1009.272 kb on - strand at 1009.395 kb on - strand, within SGL_RS06595 at 1009.395 kb on - strand, within SGL_RS06595 at 1009.724 kb on + strand, within SGL_RS06595 at 1009.852 kb on - strand, within SGL_RS06595 at 1010.014 kb on - strand, within SGL_RS06595 at 1010.060 kb on - strand, within SGL_RS06595
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 1,007,660 - SGL_RS06585 0.71 -4.1 1,007,864 + SGL_RS06585 0.81 -1.0 1,007,869 + SGL_RS06585 0.81 -2.9 1,008,187 - -3.0 1,008,438 + SGL_RS06590 0.14 -3.5 1,008,438 + SGL_RS06590 0.14 -2.6 1,008,647 - SGL_RS06590 0.39 -1.5 1,008,724 - SGL_RS06590 0.49 -3.6 1,008,756 + SGL_RS06590 0.53 -3.0 1,008,842 + SGL_RS06590 0.63 -3.6 1,008,866 + SGL_RS06590 0.66 -3.6 1,008,886 - SGL_RS06590 0.68 -2.6 1,008,991 + SGL_RS06590 0.81 -2.4 1,008,999 - SGL_RS06590 0.82 -2.0 1,009,002 + SGL_RS06590 0.82 -4.2 1,009,200 - -0.2 1,009,272 - -0.8 1,009,272 - -2.4 1,009,395 - SGL_RS06595 0.12 -2.2 1,009,395 - SGL_RS06595 0.12 -3.6 1,009,724 + SGL_RS06595 0.37 -0.5 1,009,852 - SGL_RS06595 0.46 -1.4 1,010,014 - SGL_RS06595 0.58 -2.6 1,010,060 - SGL_RS06595 0.62 -2.0
Or see this region's nucleotide sequence