Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04570 and SGL_RS04575 are separated by 11 nucleotides SGL_RS04575 and SGL_RS04580 are separated by 63 nucleotides SGL_RS04580 and SGL_RS04585 are separated by 90 nucleotides SGL_RS04585 and SGL_RS04590 are separated by 33 nucleotides
SGL_RS04570: SGL_RS04570 - Uma2 family endonuclease, at 575,477 to 576,127
_RS04570
SGL_RS04575: SGL_RS04575 - hypothetical protein, at 576,139 to 576,870
_RS04575
SGL_RS04580: SGL_RS04580 - DNA topoisomerase (ATP-hydrolyzing) subunit A, at 576,934 to 579,696
_RS04580
SGL_RS04585: SGL_RS04585 - response regulator, at 579,787 to 580,194
_RS04585
SGL_RS04590: SGL_RS04590 - zinc-dependent peptidase, at 580,228 to 581,055
_RS04590
Position (kb) 576
577
578
579
580 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 575.967 kb on - strand, within SGL_RS04570 at 576.156 kb on + strand at 576.164 kb on - strand at 576.170 kb on + strand at 576.170 kb on + strand at 576.178 kb on - strand at 576.291 kb on - strand, within SGL_RS04575 at 576.293 kb on + strand, within SGL_RS04575 at 576.421 kb on - strand, within SGL_RS04575 at 576.512 kb on + strand, within SGL_RS04575 at 576.584 kb on - strand, within SGL_RS04575 at 576.595 kb on + strand, within SGL_RS04575 at 576.637 kb on - strand, within SGL_RS04575 at 576.639 kb on + strand, within SGL_RS04575 at 576.658 kb on - strand, within SGL_RS04575 at 576.731 kb on + strand, within SGL_RS04575 at 576.872 kb on - strand at 576.932 kb on - strand at 577.015 kb on - strand at 577.033 kb on + strand at 577.046 kb on - strand at 577.092 kb on - strand at 577.209 kb on - strand at 577.342 kb on + strand, within SGL_RS04580 at 577.712 kb on + strand, within SGL_RS04580 at 577.829 kb on + strand, within SGL_RS04580 at 577.976 kb on + strand, within SGL_RS04580 at 578.009 kb on - strand, within SGL_RS04580 at 578.904 kb on + strand, within SGL_RS04580 at 579.178 kb on + strand, within SGL_RS04580 at 579.526 kb on + strand at 579.891 kb on + strand, within SGL_RS04585 at 579.918 kb on - strand, within SGL_RS04585 at 579.962 kb on - strand, within SGL_RS04585 at 579.979 kb on + strand, within SGL_RS04585 at 580.057 kb on + strand, within SGL_RS04585 at 580.106 kb on - strand, within SGL_RS04585 at 580.118 kb on - strand, within SGL_RS04585 at 580.197 kb on - strand at 580.507 kb on + strand, within SGL_RS04590 at 580.507 kb on + strand, within SGL_RS04590 at 580.585 kb on - strand, within SGL_RS04590 at 580.665 kb on + strand, within SGL_RS04590
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 575,967 - SGL_RS04570 0.75 +0.0 576,156 + +0.0 576,164 - +0.0 576,170 + +0.3 576,170 + +0.0 576,178 - -0.3 576,291 - SGL_RS04575 0.21 -0.3 576,293 + SGL_RS04575 0.21 -0.9 576,421 - SGL_RS04575 0.39 -0.7 576,512 + SGL_RS04575 0.51 +0.0 576,584 - SGL_RS04575 0.61 -0.4 576,595 + SGL_RS04575 0.62 -0.2 576,637 - SGL_RS04575 0.68 -0.1 576,639 + SGL_RS04575 0.68 -0.1 576,658 - SGL_RS04575 0.71 -0.3 576,731 + SGL_RS04575 0.81 -0.3 576,872 - -0.6 576,932 - -0.9 577,015 - -2.1 577,033 + -2.9 577,046 - -1.7 577,092 - -2.3 577,209 - -1.7 577,342 + SGL_RS04580 0.15 -2.4 577,712 + SGL_RS04580 0.28 -2.1 577,829 + SGL_RS04580 0.32 -1.0 577,976 + SGL_RS04580 0.38 -3.3 578,009 - SGL_RS04580 0.39 -0.2 578,904 + SGL_RS04580 0.71 -0.7 579,178 + SGL_RS04580 0.81 -1.1 579,526 + -1.7 579,891 + SGL_RS04585 0.25 -0.2 579,918 - SGL_RS04585 0.32 +0.2 579,962 - SGL_RS04585 0.43 +0.1 579,979 + SGL_RS04585 0.47 -0.1 580,057 + SGL_RS04585 0.66 +0.1 580,106 - SGL_RS04585 0.78 -0.2 580,118 - SGL_RS04585 0.81 -0.2 580,197 - +0.6 580,507 + SGL_RS04590 0.34 +0.2 580,507 + SGL_RS04590 0.34 +0.0 580,585 - SGL_RS04590 0.43 +0.4 580,665 + SGL_RS04590 0.53 -0.3
Or see this region's nucleotide sequence