Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04385 and SGL_RS04390 are separated by 157 nucleotides SGL_RS04390 and SGL_RS04395 overlap by 4 nucleotides SGL_RS04395 and SGL_RS04400 are separated by 2 nucleotides
SGL_RS04385: SGL_RS04385 - glutamate decarboxylase, at 543,468 to 544,871
_RS04385
SGL_RS04390: SGL_RS04390 - DUF433 domain-containing protein, at 545,029 to 545,253
_RS04390
SGL_RS04395: SGL_RS04395 - DUF5615 family PIN-like protein, at 545,250 to 545,588
_RS04395
SGL_RS04400: SGL_RS04400 - carbon-nitrogen hydrolase family protein, at 545,591 to 546,556
_RS04400
Position (kb) 545
546 Strain fitness (log2 ratio) -2
-1
0
1 at 544.065 kb on - strand, within SGL_RS04385 at 544.160 kb on - strand, within SGL_RS04385 at 544.228 kb on - strand, within SGL_RS04385 at 544.304 kb on + strand, within SGL_RS04385 at 544.522 kb on - strand, within SGL_RS04385 at 544.662 kb on + strand, within SGL_RS04385 at 544.794 kb on + strand at 544.842 kb on - strand at 545.078 kb on - strand, within SGL_RS04390 at 545.194 kb on + strand, within SGL_RS04390 at 545.211 kb on - strand, within SGL_RS04390 at 545.353 kb on + strand, within SGL_RS04395 at 545.388 kb on + strand, within SGL_RS04395 at 545.516 kb on + strand, within SGL_RS04395 at 545.546 kb on + strand, within SGL_RS04395 at 545.546 kb on + strand, within SGL_RS04395 at 545.613 kb on + strand at 545.621 kb on - strand at 545.672 kb on + strand at 545.672 kb on + strand at 545.792 kb on - strand, within SGL_RS04400 at 545.885 kb on - strand, within SGL_RS04400 at 545.896 kb on + strand, within SGL_RS04400 at 545.997 kb on - strand, within SGL_RS04400
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 544,065 - SGL_RS04385 0.43 +0.3 544,160 - SGL_RS04385 0.49 +0.1 544,228 - SGL_RS04385 0.54 +0.0 544,304 + SGL_RS04385 0.60 +0.3 544,522 - SGL_RS04385 0.75 +0.1 544,662 + SGL_RS04385 0.85 -0.2 544,794 + -0.1 544,842 - -0.5 545,078 - SGL_RS04390 0.22 -2.3 545,194 + SGL_RS04390 0.73 -1.7 545,211 - SGL_RS04390 0.81 -1.2 545,353 + SGL_RS04395 0.30 -0.3 545,388 + SGL_RS04395 0.41 +0.1 545,516 + SGL_RS04395 0.78 -0.0 545,546 + SGL_RS04395 0.87 -0.3 545,546 + SGL_RS04395 0.87 +0.1 545,613 + +0.0 545,621 - -0.2 545,672 + +0.5 545,672 + -0.2 545,792 - SGL_RS04400 0.21 -0.6 545,885 - SGL_RS04400 0.30 +0.1 545,896 + SGL_RS04400 0.32 +0.1 545,997 - SGL_RS04400 0.42 +0.4
Or see this region's nucleotide sequence