Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04140 and SGL_RS04145 are separated by 102 nucleotides SGL_RS04145 and SGL_RS04150 overlap by 11 nucleotides SGL_RS04150 and SGL_RS04155 are separated by 129 nucleotides SGL_RS04155 and SGL_RS04160 are separated by 268 nucleotides
SGL_RS04140: SGL_RS04140 - hypothetical protein, at 474,789 to 475,007
_RS04140
SGL_RS04145: SGL_RS04145 - DUF86 domain-containing protein, at 475,110 to 475,445
_RS04145
SGL_RS04150: SGL_RS04150 - nucleotidyltransferase family protein, at 475,435 to 475,743
_RS04150
SGL_RS04155: SGL_RS04155 - reverse transcriptase domain-containing protein, at 475,873 to 477,399
_RS04155
SGL_RS04160: SGL_RS04160 - glutamate synthase large subunit, at 477,668 to 482,320
_RS04160
Position (kb) 475
476
477
478 Strain fitness (log2 ratio) -2
-1
0
1 at 476.721 kb on - strand, within SGL_RS04155 at 476.737 kb on + strand, within SGL_RS04155 at 476.933 kb on + strand, within SGL_RS04155 at 476.954 kb on + strand, within SGL_RS04155 at 477.997 kb on - strand at 478.235 kb on - strand, within SGL_RS04160 at 478.236 kb on - strand, within SGL_RS04160 at 478.237 kb on - strand, within SGL_RS04160 at 478.323 kb on - strand, within SGL_RS04160 at 478.325 kb on - strand, within SGL_RS04160
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 476,721 - SGL_RS04155 0.56 -1.8 476,737 + SGL_RS04155 0.57 -1.3 476,933 + SGL_RS04155 0.69 -1.2 476,954 + SGL_RS04155 0.71 -1.1 477,997 - +0.2 478,235 - SGL_RS04160 0.12 -0.1 478,236 - SGL_RS04160 0.12 -0.3 478,237 - SGL_RS04160 0.12 -0.2 478,323 - SGL_RS04160 0.14 -0.0 478,325 - SGL_RS04160 0.14 +0.0
Or see this region's nucleotide sequence