Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03530 and SGL_RS03535 are separated by 70 nucleotides SGL_RS03535 and SGL_RS03540 are separated by 161 nucleotides SGL_RS03540 and SGL_RS03545 overlap by 4 nucleotides SGL_RS03545 and SGL_RS03550 are separated by 0 nucleotides
SGL_RS03530: SGL_RS03530 - DUF29 domain-containing protein, at 351,036 to 351,467
_RS03530
SGL_RS03535: SGL_RS03535 - Uma2 family endonuclease, at 351,538 to 352,101
_RS03535
SGL_RS03540: SGL_RS03540 - ABC transporter ATP-binding protein, at 352,263 to 353,555
_RS03540
SGL_RS03545: SGL_RS03545 - glucose-1-phosphate cytidylyltransferase, at 353,552 to 354,322
_RS03545
SGL_RS03550: SGL_RS03550 - CDP-glucose 4,6-dehydratase, at 354,323 to 355,405
_RS03550
Position (kb)
352
353
354 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 351.305 kb on - strand, within SGL_RS03530 at 351.315 kb on - strand, within SGL_RS03530 at 351.315 kb on - strand, within SGL_RS03530 at 351.328 kb on - strand, within SGL_RS03530 at 351.520 kb on + strand at 351.520 kb on + strand at 351.922 kb on + strand, within SGL_RS03535 at 351.966 kb on - strand, within SGL_RS03535 at 352.226 kb on - strand at 352.324 kb on + strand at 352.334 kb on + strand at 352.336 kb on + strand at 352.422 kb on - strand, within SGL_RS03540 at 352.479 kb on - strand, within SGL_RS03540 at 352.479 kb on - strand, within SGL_RS03540 at 352.505 kb on + strand, within SGL_RS03540 at 352.537 kb on + strand, within SGL_RS03540 at 352.659 kb on + strand, within SGL_RS03540 at 352.670 kb on + strand, within SGL_RS03540 at 353.059 kb on + strand, within SGL_RS03540 at 353.061 kb on + strand, within SGL_RS03540 at 353.481 kb on + strand at 353.486 kb on + strand at 353.820 kb on - strand, within SGL_RS03545 at 354.014 kb on + strand, within SGL_RS03545 at 354.213 kb on - strand, within SGL_RS03545 at 354.409 kb on - strand at 354.459 kb on + strand, within SGL_RS03550 at 354.468 kb on - strand, within SGL_RS03550 at 354.504 kb on + strand, within SGL_RS03550
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 351,305 - SGL_RS03530 0.62 +0.1 351,315 - SGL_RS03530 0.65 +0.2 351,315 - SGL_RS03530 0.65 -0.1 351,328 - SGL_RS03530 0.68 -0.4 351,520 + +0.4 351,520 + -0.1 351,922 + SGL_RS03535 0.68 -1.1 351,966 - SGL_RS03535 0.76 -0.2 352,226 - +0.0 352,324 + -1.8 352,334 + -1.6 352,336 + -1.8 352,422 - SGL_RS03540 0.12 -2.0 352,479 - SGL_RS03540 0.17 -3.1 352,479 - SGL_RS03540 0.17 -3.1 352,505 + SGL_RS03540 0.19 -2.8 352,537 + SGL_RS03540 0.21 -1.9 352,659 + SGL_RS03540 0.31 -0.9 352,670 + SGL_RS03540 0.31 -1.9 353,059 + SGL_RS03540 0.62 -1.2 353,061 + SGL_RS03540 0.62 -0.7 353,481 + -0.1 353,486 + -0.9 353,820 - SGL_RS03545 0.35 -0.7 354,014 + SGL_RS03545 0.60 -0.3 354,213 - SGL_RS03545 0.86 -0.0 354,409 - +0.2 354,459 + SGL_RS03550 0.13 +0.2 354,468 - SGL_RS03550 0.13 +0.7 354,504 + SGL_RS03550 0.17 -0.4
Or see this region's nucleotide sequence