Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03280 and SGL_RS03285 are separated by 12 nucleotides SGL_RS03285 and SGL_RS03290 overlap by 4 nucleotides SGL_RS03290 and SGL_RS03295 are separated by 233 nucleotides
SGL_RS03280: SGL_RS03280 - FAD-dependent hydroxylase, at 301,110 to 302,354
_RS03280
SGL_RS03285: SGL_RS03285 - fluoride efflux transporter CrcB, at 302,367 to 302,789
_RS03285
SGL_RS03290: SGL_RS03290 - LapA family protein, at 302,786 to 303,190
_RS03290
SGL_RS03295: SGL_RS03295 - hypothetical protein, at 303,424 to 303,621
_RS03295
Position (kb) 302
303 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 302.354 kb on + strand at 302.410 kb on - strand, within SGL_RS03285 at 302.440 kb on - strand, within SGL_RS03285 at 302.459 kb on - strand, within SGL_RS03285 at 302.489 kb on + strand, within SGL_RS03285 at 302.497 kb on - strand, within SGL_RS03285 at 302.511 kb on - strand, within SGL_RS03285 at 302.550 kb on + strand, within SGL_RS03285 at 302.686 kb on - strand, within SGL_RS03285 at 302.973 kb on - strand, within SGL_RS03290 at 303.064 kb on - strand, within SGL_RS03290 at 303.142 kb on + strand, within SGL_RS03290 at 303.344 kb on - strand at 303.403 kb on - strand at 303.648 kb on - strand at 303.710 kb on - strand at 303.732 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 302,354 + -0.0 302,410 - SGL_RS03285 0.10 +1.8 302,440 - SGL_RS03285 0.17 -0.1 302,459 - SGL_RS03285 0.22 -0.3 302,489 + SGL_RS03285 0.29 +0.0 302,497 - SGL_RS03285 0.31 -0.2 302,511 - SGL_RS03285 0.34 -0.0 302,550 + SGL_RS03285 0.43 +0.0 302,686 - SGL_RS03285 0.75 +0.9 302,973 - SGL_RS03290 0.46 -3.5 303,064 - SGL_RS03290 0.69 -0.5 303,142 + SGL_RS03290 0.88 +0.1 303,344 - -2.9 303,403 - -0.9 303,648 - +0.2 303,710 - -0.3 303,732 - +0.1
Or see this region's nucleotide sequence