Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02550 and SGL_RS02555 are separated by 0 nucleotides SGL_RS02555 and SGL_RS02560 are separated by 205 nucleotides SGL_RS02560 and SGL_RS02565 are separated by 138 nucleotides
SGL_RS02550: SGL_RS02550 - agmatinase, at 148,022 to 148,942
_RS02550
SGL_RS02555: SGL_RS02555 - peptidylprolyl isomerase, at 148,943 to 149,683
_RS02555
SGL_RS02560: SGL_RS02560 - photosystem I assembly protein Ycf4, at 149,889 to 150,455
_RS02560
SGL_RS02565: SGL_RS02565 - GH3 auxin-responsive promoter family protein, at 150,594 to 152,072
_RS02565
Position (kb)
149
150
151 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 149.089 kb on + strand, within SGL_RS02555 at 149.125 kb on - strand, within SGL_RS02555 at 149.505 kb on + strand, within SGL_RS02555 at 149.570 kb on + strand, within SGL_RS02555 at 149.717 kb on + strand at 149.873 kb on + strand at 149.972 kb on - strand, within SGL_RS02560 at 150.022 kb on - strand, within SGL_RS02560 at 150.250 kb on + strand, within SGL_RS02560 at 150.510 kb on + strand at 150.510 kb on + strand at 150.518 kb on - strand at 150.548 kb on - strand at 150.563 kb on - strand at 150.682 kb on + strand at 150.697 kb on + strand at 150.697 kb on + strand at 150.705 kb on - strand at 150.705 kb on - strand at 150.946 kb on - strand, within SGL_RS02565 at 150.953 kb on + strand, within SGL_RS02565 at 151.042 kb on + strand, within SGL_RS02565 at 151.410 kb on + strand, within SGL_RS02565 at 151.418 kb on - strand, within SGL_RS02565
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 149,089 + SGL_RS02555 0.20 -1.4 149,125 - SGL_RS02555 0.25 -2.6 149,505 + SGL_RS02555 0.76 -1.6 149,570 + SGL_RS02555 0.85 -2.4 149,717 + +0.1 149,873 + +0.2 149,972 - SGL_RS02560 0.15 -1.0 150,022 - SGL_RS02560 0.23 -3.5 150,250 + SGL_RS02560 0.64 -2.6 150,510 + -0.1 150,510 + +0.1 150,518 - +0.1 150,548 - +0.2 150,563 - -0.1 150,682 + -0.2 150,697 + +0.3 150,697 + -0.0 150,705 - -0.1 150,705 - +0.0 150,946 - SGL_RS02565 0.24 -0.2 150,953 + SGL_RS02565 0.24 +0.6 151,042 + SGL_RS02565 0.30 -1.4 151,410 + SGL_RS02565 0.55 -0.1 151,418 - SGL_RS02565 0.56 -0.6
Or see this region's nucleotide sequence