Experiment: Cobalt chloride 5 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01895 and SGL_RS01900 are separated by 182 nucleotides SGL_RS01900 and SGL_RS01905 are separated by 144 nucleotides
SGL_RS01895: SGL_RS01895 - magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase, at 3,192 to 4,268
_RS01895
SGL_RS01900: SGL_RS01900 - GDP-L-fucose synthase, at 4,451 to 5,389
_RS01900
SGL_RS01905: SGL_RS01905 - GDP-mannose 4,6-dehydratase, at 5,534 to 6,622
_RS01905
Position (kb) 4
5
6 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4.628 kb on + strand, within SGL_RS01900 at 4.682 kb on - strand, within SGL_RS01900 at 4.878 kb on - strand, within SGL_RS01900 at 4.916 kb on + strand, within SGL_RS01900 at 5.037 kb on - strand, within SGL_RS01900 at 5.178 kb on - strand, within SGL_RS01900 at 5.178 kb on - strand, within SGL_RS01900 at 5.198 kb on + strand, within SGL_RS01900 at 5.237 kb on + strand, within SGL_RS01900 at 5.571 kb on + strand at 5.760 kb on - strand, within SGL_RS01905 at 5.873 kb on + strand, within SGL_RS01905 at 5.917 kb on + strand, within SGL_RS01905 at 5.918 kb on - strand, within SGL_RS01905 at 5.936 kb on - strand, within SGL_RS01905 at 6.026 kb on + strand, within SGL_RS01905 at 6.026 kb on + strand, within SGL_RS01905 at 6.119 kb on + strand, within SGL_RS01905 at 6.132 kb on + strand, within SGL_RS01905 at 6.137 kb on + strand, within SGL_RS01905 at 6.145 kb on - strand, within SGL_RS01905 at 6.145 kb on - strand, within SGL_RS01905 at 6.246 kb on + strand, within SGL_RS01905 at 6.355 kb on - strand, within SGL_RS01905
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 5 uM remove 4,628 + SGL_RS01900 0.19 -2.8 4,682 - SGL_RS01900 0.25 -2.3 4,878 - SGL_RS01900 0.45 -1.2 4,916 + SGL_RS01900 0.50 -2.0 5,037 - SGL_RS01900 0.62 -1.4 5,178 - SGL_RS01900 0.77 -3.4 5,178 - SGL_RS01900 0.77 -2.3 5,198 + SGL_RS01900 0.80 -2.9 5,237 + SGL_RS01900 0.84 -2.4 5,571 + -1.2 5,760 - SGL_RS01905 0.21 -3.4 5,873 + SGL_RS01905 0.31 -4.0 5,917 + SGL_RS01905 0.35 -2.5 5,918 - SGL_RS01905 0.35 -2.1 5,936 - SGL_RS01905 0.37 -1.4 6,026 + SGL_RS01905 0.45 -0.3 6,026 + SGL_RS01905 0.45 -2.9 6,119 + SGL_RS01905 0.54 -3.0 6,132 + SGL_RS01905 0.55 -3.2 6,137 + SGL_RS01905 0.55 -3.3 6,145 - SGL_RS01905 0.56 -2.4 6,145 - SGL_RS01905 0.56 -1.6 6,246 + SGL_RS01905 0.65 -1.1 6,355 - SGL_RS01905 0.75 -1.7
Or see this region's nucleotide sequence