Experiment: Cobalt chloride 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16085 and SGL_RS16090 are separated by 129 nucleotides SGL_RS16090 and SGL_RS16095 are separated by 11 nucleotides SGL_RS16095 and SGL_RS16100 are separated by 151 nucleotides
SGL_RS16085: SGL_RS16085 - heavy metal translocating P-type ATPase, at 3,058,411 to 3,060,339
_RS16085
SGL_RS16090: SGL_RS16090 - YnfA family protein, at 3,060,469 to 3,060,795
_RS16090
SGL_RS16095: SGL_RS16095 - Zn(II)-sensing metalloregulatory transcriptional repressor ZiaR, at 3,060,807 to 3,061,205
_RS16095
SGL_RS16100: SGL_RS16100 - heavy metal translocating P-type ATPase, at 3,061,357 to 3,063,522
_RS16100
Position (kb)
3060
3061
3062 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 3059.908 kb on - strand, within SGL_RS16085 at 3059.930 kb on - strand, within SGL_RS16085 at 3059.945 kb on - strand, within SGL_RS16085 at 3060.016 kb on + strand, within SGL_RS16085 at 3060.043 kb on + strand, within SGL_RS16085 at 3060.091 kb on + strand, within SGL_RS16085 at 3060.151 kb on - strand at 3060.264 kb on + strand at 3060.309 kb on + strand at 3060.475 kb on - strand at 3060.475 kb on - strand at 3060.506 kb on + strand, within SGL_RS16090 at 3060.519 kb on - strand, within SGL_RS16090 at 3060.519 kb on - strand, within SGL_RS16090 at 3060.522 kb on + strand, within SGL_RS16090 at 3060.530 kb on - strand, within SGL_RS16090 at 3060.530 kb on - strand, within SGL_RS16090 at 3060.530 kb on - strand, within SGL_RS16090 at 3060.575 kb on + strand, within SGL_RS16090 at 3060.699 kb on + strand, within SGL_RS16090 at 3060.708 kb on - strand, within SGL_RS16090 at 3060.884 kb on - strand, within SGL_RS16095 at 3060.892 kb on - strand, within SGL_RS16095 at 3060.941 kb on + strand, within SGL_RS16095 at 3061.014 kb on + strand, within SGL_RS16095 at 3061.035 kb on + strand, within SGL_RS16095 at 3061.076 kb on + strand, within SGL_RS16095 at 3061.092 kb on + strand, within SGL_RS16095 at 3061.111 kb on + strand, within SGL_RS16095 at 3061.128 kb on - strand, within SGL_RS16095 at 3061.180 kb on + strand at 3061.209 kb on + strand at 3061.402 kb on + strand at 3061.468 kb on - strand at 3061.468 kb on - strand at 3061.468 kb on - strand at 3061.478 kb on - strand at 3061.502 kb on + strand at 3061.522 kb on - strand at 3061.522 kb on - strand at 3061.524 kb on + strand at 3061.524 kb on + strand at 3061.571 kb on - strand at 3061.571 kb on - strand at 3061.599 kb on + strand, within SGL_RS16100 at 3061.606 kb on - strand, within SGL_RS16100 at 3061.613 kb on + strand, within SGL_RS16100 at 3061.646 kb on + strand, within SGL_RS16100 at 3061.648 kb on - strand, within SGL_RS16100 at 3061.803 kb on + strand, within SGL_RS16100 at 3061.809 kb on + strand, within SGL_RS16100 at 3061.809 kb on + strand, within SGL_RS16100 at 3061.811 kb on + strand, within SGL_RS16100 at 3061.829 kb on - strand, within SGL_RS16100 at 3061.918 kb on - strand, within SGL_RS16100 at 3061.971 kb on + strand, within SGL_RS16100 at 3062.021 kb on - strand, within SGL_RS16100 at 3062.049 kb on + strand, within SGL_RS16100 at 3062.083 kb on + strand, within SGL_RS16100 at 3062.163 kb on + strand, within SGL_RS16100
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 3 uM remove 3,059,908 - SGL_RS16085 0.78 -3.1 3,059,930 - SGL_RS16085 0.79 -3.6 3,059,945 - SGL_RS16085 0.80 -3.4 3,060,016 + SGL_RS16085 0.83 -5.7 3,060,043 + SGL_RS16085 0.85 -2.4 3,060,091 + SGL_RS16085 0.87 -2.6 3,060,151 - -3.5 3,060,264 + -2.8 3,060,309 + -2.6 3,060,475 - +0.4 3,060,475 - +0.1 3,060,506 + SGL_RS16090 0.11 +0.2 3,060,519 - SGL_RS16090 0.15 +0.1 3,060,519 - SGL_RS16090 0.15 +0.1 3,060,522 + SGL_RS16090 0.16 +0.5 3,060,530 - SGL_RS16090 0.19 -0.0 3,060,530 - SGL_RS16090 0.19 +0.2 3,060,530 - SGL_RS16090 0.19 +0.0 3,060,575 + SGL_RS16090 0.32 -0.2 3,060,699 + SGL_RS16090 0.70 +0.2 3,060,708 - SGL_RS16090 0.73 -0.1 3,060,884 - SGL_RS16095 0.19 +1.2 3,060,892 - SGL_RS16095 0.21 +0.7 3,060,941 + SGL_RS16095 0.34 -0.0 3,061,014 + SGL_RS16095 0.52 -0.0 3,061,035 + SGL_RS16095 0.57 -0.1 3,061,076 + SGL_RS16095 0.67 -0.1 3,061,092 + SGL_RS16095 0.71 -0.3 3,061,111 + SGL_RS16095 0.76 -0.2 3,061,128 - SGL_RS16095 0.80 +0.0 3,061,180 + -0.6 3,061,209 + -0.1 3,061,402 + -2.6 3,061,468 - -3.3 3,061,468 - -2.3 3,061,468 - -2.8 3,061,478 - -3.3 3,061,502 + -3.2 3,061,522 - -2.8 3,061,522 - -1.6 3,061,524 + -4.7 3,061,524 + -3.0 3,061,571 - -3.3 3,061,571 - -4.5 3,061,599 + SGL_RS16100 0.11 -2.3 3,061,606 - SGL_RS16100 0.11 -3.3 3,061,613 + SGL_RS16100 0.12 -1.6 3,061,646 + SGL_RS16100 0.13 -3.0 3,061,648 - SGL_RS16100 0.13 -4.4 3,061,803 + SGL_RS16100 0.21 -3.4 3,061,809 + SGL_RS16100 0.21 -3.7 3,061,809 + SGL_RS16100 0.21 -3.4 3,061,811 + SGL_RS16100 0.21 -3.8 3,061,829 - SGL_RS16100 0.22 -1.6 3,061,918 - SGL_RS16100 0.26 -5.4 3,061,971 + SGL_RS16100 0.28 -3.3 3,062,021 - SGL_RS16100 0.31 -4.6 3,062,049 + SGL_RS16100 0.32 -3.0 3,062,083 + SGL_RS16100 0.34 -5.0 3,062,163 + SGL_RS16100 0.37 -1.6
Or see this region's nucleotide sequence