Experiment: Cobalt chloride 3 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09480 and SGL_RS09485 are separated by 105 nucleotides SGL_RS09485 and SGL_RS09490 overlap by 11 nucleotides SGL_RS09490 and SGL_RS19865 are separated by 69 nucleotides SGL_RS19865 and SGL_RS18860 overlap by 91 nucleotides SGL_RS18860 and SGL_RS09505 overlap by 32 nucleotides
SGL_RS09480: SGL_RS09480 - IS4-like element IS4Sa family transposase, at 1,624,643 to 1,625,095
_RS09480
SGL_RS09485: SGL_RS09485 - sugar phosphate isomerase/epimerase, at 1,625,201 to 1,625,632
_RS09485
SGL_RS09490: SGL_RS09490 - CBS domain-containing protein, at 1,625,622 to 1,626,095
_RS09490
SGL_RS19865: SGL_RS19865 - IS630 transposase-related protein, at 1,626,165 to 1,626,524
_RS19865
SGL_RS18860: SGL_RS18860 - IS630 family transposase, at 1,626,434 to 1,627,012
_RS18860
SGL_RS09505: SGL_RS09505 - DegT/DnrJ/EryC1/StrS family aminotransferase, at 1,626,981 to 1,628,018
_RS09505
Position (kb)
1625
1626
1627 Strain fitness (log2 ratio)
-1
0
1 at 1624.666 kb on - strand at 1625.182 kb on + strand at 1625.182 kb on + strand at 1625.318 kb on + strand, within SGL_RS09485 at 1625.889 kb on + strand, within SGL_RS09490 at 1625.897 kb on - strand, within SGL_RS09490 at 1625.907 kb on + strand, within SGL_RS09490 at 1626.001 kb on - strand, within SGL_RS09490 at 1626.001 kb on - strand, within SGL_RS09490
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 3 uM remove 1,624,666 - -0.6 1,625,182 + +0.4 1,625,182 + -0.1 1,625,318 + SGL_RS09485 0.27 -0.1 1,625,889 + SGL_RS09490 0.56 +1.3 1,625,897 - SGL_RS09490 0.58 +0.5 1,625,907 + SGL_RS09490 0.60 +0.0 1,626,001 - SGL_RS09490 0.80 +0.5 1,626,001 - SGL_RS09490 0.80 +0.3
Or see this region's nucleotide sequence